诊断用超声对宫内早孕绒毛影响的多指标评价

MultiTarget Evaluation of Effects on Human Early Pregnancy Villous in Uteri after Exposed to Diagnostic Ultrasound

目的：为了客观评价诊断用超声对宫内早孕绒毛有无影响，了解其量效关系以及损伤能否逆转。方法：应用日本产ＥＵＢ２００型实时超声诊断仪，探头频率３．５ＭＨｚ。将１２５正常早孕妇女随机分成正常对照组（Ⅰ组）、照射１０分钟（Ⅱ组）、照射２０分钟（Ⅲ组）、照射３０分钟（Ⅳ组）和照３０分钟后７～１０天取材检测。各组在人工流产后取绒毛标本检测丙二醛、谷胱甘肽过氧化酶、ＤＮＡ含量、染色体畸变率和绒毛细胞的形态学改变。结果：照射２０分钟丙二醛含量增加，谷胱甘肽过氧化酶活性下降，照射３０分钟ＤＮＡ含量下降。上述改变于７～１０天后基本恢复至正常对照组水平。染色体畸变率及绒毛细胞形态学各照射组与正常对照组无显著性差异。结论：诊断用超声对宫内早孕绒毛细胞的生化代谢和分子水平的损伤与照射时间有关，照射３０分钟以内所造成的损伤经７～１０天可恢复至正常水平。诊断用超声对早孕妇女照射１０分钟以内是安全的。

Subject:To study whether the diagnostic ultrasound (DU)damage the human early pregnancy villous, to understand the relationship between quantity and effect of DU and whether the damage changes can become normal.\ [WTHZ]Methods:125 early pregnancy woman were random divided into five groups Ⅰ,Ⅱ,Ⅲ,Ⅳ and Ⅴ which were exposed to DU for 0, 10,20,30 and 30 minutes respectively with EUB 200, made in Japan(probe 3.5MHz).Patientsvillous samples of groups ⅠⅣ were collected soon, while V were collected after exposed 710 days. The MDA, GSHpx,DNA content ,chromosome aberration rate and the cytomorphology changes were analyzed.Results:MDA and GSHpx of human early pregnancy in uteri were increased and decreased respectively (P<0.01) for exposed to DU 20 minutes . The DNA content was decreased for exposed 30 minutes . These changes became normal after 710 days (group V).While there were no significant changes in chromosome aberration rate and the cytomorphology (P>0.05).Conclusion:Cytobiochemical metabolism changes and molecular damages of human early pregnancy villous resulted from DU refer to exposure time . The changes exposed exposed shorter than 30 minutes became normal within 7-10 days. For early pregnancy woman, being exposed to DU shorter than 10 minutes is safe .