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成纤维细胞与结肠癌细胞相互作用对IGFBP7表达的影响 被引量：1

Fibroblasts-colorectal cancer cells interaction induces the expression of IGFBP7
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摘要 目的:试图证实结肠癌细胞与成纤维细胞相互作用能影响胰岛素样生长因子结合蛋白7(IGFBP7)的表达,初步探讨肿瘤-间质相互作用对结肠癌生长的作用。方法:结肠癌细胞系SW480与成纤维细胞HELF共培养于RPMI1640培养液中,分别于0、48、72和96h收集细胞,采用RT-PCR和免疫细胞化学法检测两种细胞中IGFBP7的表达。结果:两种细胞经共培养48h后,SW480细胞IGFBP7 mRNA和蛋白表达均上调,HELF细胞原不表达IGFBP7,经与肿瘤细胞共培养48h后,开始表达IGFBP7并与共培养时间成正相关。结论:结肠癌细胞与成纤维细胞相互作用促进二者表达IGFBP7,提示肿瘤-间质相互作用可能对结肠癌演进具有抑制作用。 Objective： To investigate the impact of intracellular interaction between fibroblasts and colorectal cancer ceils on the expression of insulin growth factor binding protein7 （IGFBP7）. Methods： Colorectal cancer ceils SW480 were cultured with or without fibroblasts HELF cells in RPMI 1640 medium for 0 h, 48 h, 72 h and 96 h, respectively. By RT-PCR and immunohistochemical staining methods,the expression of IGFBP7 was detected in mono- and cocultured cells. Results： When SW480 cells were co-cultured with HELF cells,IGFBP7 expression in SW480 cells was significantly upregulated. Furthermore,IGFBP7 was induced in HELF cells both at mRNA and protein levels, which did not express when cells were mono-cultured. Conclusion. Fibroblasts-colorectal cancer cells interaction induces the expression of IGFBPT, which indicates tumor-stroma interactions may play an important role in colorectal cancer development.
作者 申秀锦 张有为 邓红 陈露露 陈平圣
机构地区 浙江大学医学院病理学与病理生理学系 南京大学医学院附属南京军区总医院肿瘤科 东南大学基础医学院病理学与病理生理学系
出处 《浙江大学学报（医学版）》 CAS CSCD 北大核心 2009年第2期151-157,共7页 Journal of Zhejiang University(Medical Sciences)
基金 浙江省自然科学基金(Y207308) 浙江省教育厅基金(20070132)
关键词 结肠肿瘤 成纤维细胞 胰岛素样生长因子结合蛋白质类 IGFBP7 细胞间相互作用 共培养 结肠癌细胞 Colonic neoplasms Fibroblasts Insulin-like growth factor binding proteins IGFBP7 Intercellular interaction Co-culture Colorectal cancer
分类号 R735.35 [医药卫生—肿瘤]
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参考文献24

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共引文献16

同被引文献19

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浙江大学学报（医学版）
2009年 第2期

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