摘要
目的:观察BCG对变态反应性鼻炎小鼠的TH细胞免疫的影响并探讨其机制。方法:30只昆明小白鼠随机分为对照组,变态反应性鼻炎组及干预组,干预组造模前及后5d分别以BCG皮内注射干预,造模2周后采用流式细胞仪分析测定外周血Th1/Th2细胞之比,ELISA试剂盒IL.4、IL-10、IFN-γ水平。结果:造模组外周血中IL-4、 IL-10较正常对照组明显升高,而IFN-γ水平降低(P<0.05);与造模组相比,治疗组外周血中IL-4、IL-10水平下降, IFN-γ水平升高(P<0.05);造模组外周血Th1/Th2比值较正常对照组下降(P<0.05);与造模组比较,干预组Th1/Th2比值升高(P<0.05)。结论:Th1/Th2失平衡是变应性鼻炎发病的重要机理,BCG能调节变态反应性鼻炎小鼠的T细胞免疫,干预变应性鼻炎的发病。
Objective：To explore Effects of Bacillus Calmette-Guerin on Th cell immunity in Mouse of allergic rhinitis and research its principle.Methods：30 mouses were divided into three groups：control group、allergic rhinitis group、BCG interfereing group.Methods： received intradermal injection with BCG before making allergic rhinitis and after five days.Then the peripheral blood Th1/Th2 ratio was detected by using flow cytometry method and IL-4,IL-10 and IFN-γin the peripheral blood were analysed by ELISA.Results：Compared with the control group,allergic rhinitis group group has higher IL-4and IL-10 level and lower IFN-γlevel in the peripheral blood,P〈0.05.Compared with allergic rhinitis group,BCG interfereing group has lower IL-4 level and higher IFN-γlevel in the peripheral blood,P〈0.05.Blood Th1/Th2 cells ratio in the allergic rhinitis group was lower compared with control group,P〈0.05.Compared with allergic rhinitis group BCG interfereing group has higher ratio of Th1/Th2 cells in the peripheral blood,P〉0.05.Conclusion： The immune imbalance of Th1/Th2 is a important role in allergic rhinitis,BCG can adjust Th cell immunity in Mouse of allergic rhinitis,interfereing morbidity of allergic rhinitis.
出处
《中国医药导刊》
2009年第1期94-95,共2页
Chinese Journal of Medicinal Guide
