吖啶橙与牛血清白蛋白的相互结合反应

The Interaction between Acridine Orange and Bovine Serum Albumin

应用光谱法研究了水溶液体系中吖啶橙与牛血清白蛋白分子间的相互结合反应，其 结合常数为ｋ＝４．８×１０３ｍｏｌ／Ｌ，结合位点数为ｎ＝０．２８。并依据Ｆｏｒｓｔｅｒ非辐射能量转移机 制，探讨了吖啶橙与牛血清白蛋白相互结合时，其给体－受体间距离（Ｒｏ＝１．５ ｎｍ）和能量转移 效率（Ｅ＝０．３２）。证实了吖啶橙与牛血清白蛋白的相互结合作用为单一的静态猝灭过程，且 阐明了其猝灭机制是通过能量转移产生的。

We report the results on the binding characteristics of acridine orange (AO) and bovine serum albumin (BSA) by measuring fluorescence spectra. The equilibrium constant k was found to be 4. 8 ×103 L/mol, and the number of binding sites n=0. 82. Based on the mechanism of Forster energy transfer, the transfer efficiency of energy and distance between acceptor AO and BSA were obtained. The interaction between AO and BSA have been veri- fied as consistent with static quenching procedure and the quenching mechanism is related to the energy transfer.