我国8城市HBsAg阳性和阴性乙肝患者的病毒血清型和基因型分析

Serotyping and genotyping of hepatitis B virus among HBsAg positive and negative hepatitis B patients in 8 cities of China

目的对我国８个城市的４３株乙型肝炎表面抗原（ＨＢｓＡｇ）阳性和５２株ＨＢｓＡｇ阴性血清进行乙肝病毒（ＨＢＶ）ＤＮＡ扩增及其血清型和基因型分型，并对该两类血清的乙肝病毒型别构成及其地理分布进行比较。方法对长春、大同、杭州、深圳、青岛、西安、昆明和拉萨等８个城市共计４３例ＨＢ－ｓＡｇ阳性和５２例ＨＢｓＡｇ阴性的乙肝病人血清进行ＨＢＶＤＮＡＰＣＲ扩增（Ｓ基因第３３～５３３位核苷酸）并用ＰＣＲ扩增产物直接测序法分析ＨＢＶ血清型和基因型。结果ＨＢｓＡｇ阳性和阴性乙肝的ＨＢＶ血清型均以ａｄｒｑ＋为最多，分别为５８．１％和４０％；其次是ａｄｗ２，分别为２５．６％和３２．７％。ＨＢｓＡｇ阴性乙肝组ａｙｗ３（１９．２％）明显多于ＨＢｓＡｇ阳性乙肝组（２．３％，Ｐ＜０．０５）；而ａｙｗ１前者（０％）显著少于后者（１１．６％，Ｐ＜０．０１）。ＨＢｓＡｇ阳性和阴性乙肝的ＨＢＶ基因型均以Ｃ型为主，分别为６２．８％和６１．５％；其次为Ｄ型，分别为１４％和２６．９％；Ｂ型，分别为２０．９％和９．６％；Ａ型，各１株。各城市间两类乙肝的ＨＢＶ血清型和基因型存在一定差异。总体上看，两类乙肝的ＨＢＶ主要型别构成基本一致。结论ＨＢ－ｓＡｇ阴性乙肝?

By polymerase chain reaction (PCR)and direct sequencing of HBV DNA products (53nd～513th nucleotides of S gene),the serotypes and genotypes of HBV in sera of 43 HBsAg positive &52 HBsAg negative HB patients enrolled from Changchun, Datong,Hangzhou,Shenzhen,Qingdao,Xian,Kunming and Lasa were determined.Comparison of the serotypes and genotypes of hepatitis B virus (HBV) between HBsAg negative and positive Hepatitis B (HB) shown that adrq+was the most predominant serotype in both HBsAg positive and negative HB groups (58.1% and 40.0%,respectively),adw2 was the next (25.6% and 32.7%,respectively),ayw3 in the HBsAg negative HB group (19.2%) was more than in the HBsAg positive HB group(2.3%)(P<0.05)and ayw1(0%) was less than the latter (11.6%)(P<0.01).Genotype C was most frequent in both HBsAg positive and negative HB groups(62.8% and 61.5%,respectively).Genotype D (14.0% and 26.9%,respectively)and B (20.9% and 9.6%,respectively)were the next.Only 2 HBV strains of genotype A (each of the two HB groups )were identified. Some differences of HBV serotypes and genotypes among the 8 cities were observed in the two HB groups.The main HBV serotypes and genotypes of the two HB groups were similar,it indicated that low titer of HBV in blood was the major cause of HBsAg negative HB,and mutation of HBV played no significant role.