糖克宁颗粒的降糖降脂作用及其机理研究

The Study of Effection and Mechanism of Tangkening Granule on Rats Blood Glucose and Blood Fat

目的研究糖克宁颗粒降血糖、血脂的作用及其机理。方法采用静脉注射链脲佐菌素,并给以高脂饲料制备实验性糖尿病大鼠模型,以糖克宁颗粒进行治疗,观察模型大鼠的血糖、糖耐量、肝糖原、胰岛素、胰岛素样生长因子及肿瘤坏死因子,高低密度脂蛋白等的变化。结果与对照组相比,糖克宁颗粒各剂量组均可有效降低实验性糖尿病大鼠的血糖(P<0.05),增加肝糖原、胰岛素及胰岛素样生长因子,升高高密度脂蛋白,降低肿瘤坏死因子和低密度脂蛋白。结论糖克宁颗粒能有效降低实验性糖尿病大鼠的血糖、血脂,改善糖耐量,其作用机制可能是通过增加肝糖原、胰岛素,提高胰岛素敏感性及提高抗氧化性、减少脂质过氧化产物来实现对血糖和血脂的调节作用。

OBJECTIVE To investigate the effection and mechanism of Tangkening granule on rats blood glucose and blood fat. METHODS Rat diabetes models were made by injecting Steptozotocin （10 mg. kg^-1）in intravenous followed by feeding high-lipid and high-sugar diat. Then administer the rats by Tangkening granule, and observed the change of weight and detected the contents of blood glucose, carbohydrate tolerance, hepatic glycogen, Insulin and insulin-like growth factor, tumor necrosis factor, HDL-C, LDL-C and so on. RESULTS Comparating with controlling group, Tangkening granule in evrery gloup can low the blood glucose obviously on experimental diabetic rats （ P 〈 0.05 ）, can raise hepatic glycogen, HDL-C, Insulin and insulin-like growth factor and can degrade tumor necrosis factor, LDL-C. CONCLUSION The experiment results indicate that Tangkening granule can low the blood glucose and blood fat obviously, improve carbohydrate tolerance. The mechanism of controlling blood glucose and blood fat maybe related to raise hepatic glycogen and Insulin, improve Insulin Sensitivity and the ability of antioxidation, reduce the activity of MDA in the rats.