摘要
In Tris-HCI buffer an intermolecular phosphoryl group transfer from N-(O,Odiisopeyl) phosphoryl asparagine (DIPPAsn) to trypsin was confirmed The resultsuggested an novel way to phosphorylate the protein without kinase participation.
In Tris-HCI buffer an intermolecular phosphoryl group transfer from N-(O,Odiisopeyl) phosphoryl asparagine (DIPPAsn) to trypsin was confirmed The resultsuggested an novel way to phosphorylate the protein without kinase participation.
