摘要
Our previous works were done on the abnormal permeability of vasculature and cellular membrane after hemorrhagic fever with renal syndrome virus (HFRSV) infection. In this study, the internalization of viral antigens in the experimentally infected suckling BALB/c mice with Chen strain HFRSV were further studied by the application of the colloidal gold-labelled polyclonal and monoclonal antibodies against HFRSV (GLAb) to the infected animals via tail veins. The normal mice with GLAb and the infected mice with colloidal gold-labelled indifferent antibodies (GLIg) were also employed as experimental controls. At certain time intervals after the injection,the animals were sacrificed and tissues were observed under light and electron microscopes. In the normal mice,the conjugates were confined to the vasculature and reliculo-endothelial system and localized in the lysosomes of phagocytes. In the infected animals,the GLIg conjugates could be used to demonstrate the abnormal permeability,but could not show the antigen localization,while the GLAb could be internalized into the cytoplasm of the parenchymal cells and localized in the free ribosome,Golgi apparatus,granules within vesicles,and inclusion body-like structures Compared to the infected animals with GLIg,obvious tissue structure alternations under LM were observed in the infected mice with GLAb. The ultrastructural changes of destruction and abnormal structures frequently occurred in the cells of the infected animals. The typical virion,immature virion and inclusion body could be found but only in a few cells. The results indicated that the free ribosomes,Golgi apparatus and vesices may be related to viral infection and positive viral antigen could not represent the virus structure only. It is suggested that the antibodies produced in the bodies after HFRSV infection can bind not only with the extracel lular viral antigens to form immunocomplexes to induce tissue lesions but also the cytoplasmic viral antigens of the infected cells through the damaged cellular membranes.
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