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慢性乙型肝炎患者及非肝病患者肝组织中巨细胞病毒的检测

Detection of cytomegalovirus in liver tissue from patients without hepatitis and with chronic hepatitis B
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摘要 目的探讨巨细胞病毒(CMV)在非肝病患者及慢性乙型肝炎患者肝组织中的表达、分布特点及相关关系。方法对31例非肝病和76例慢性乙型肝炎患者肝组织采用免疫组织化学以抗-CMV单克隆抗体进行检测,用抗乙型肝炎核心抗原(抗-HBcAg)多克隆抗体和抗-CMV单克隆抗体双标记技术在慢性乙型肝炎患者同一张肝组织切片上显示两种病毒分布特点。结果31例非肝病肝组织中检出CMVAg 7例,明显低于慢性乙型肝炎患者(22.6%vs 56.6%,P<0.01)。在两组标本中CMVAg均在肝细胞胞浆内表达。非肝病患者肝组织中阳性细胞数量较慢性乙型肝炎患者少;后者阳性细胞多近汇管区分布,数量多时也可在肝小叶内弥漫分布。轻度和重度慢性肝炎病例中CMV的检出率差异无统计学意义(P>0.05)。双标记染色显示CMVAg和HBcAg多数可表达于肝小叶内同一区域肝细胞甚至同一肝细胞内,也可见于肝小叶中不同部位。结论非肝病患者中CMV感染并不少见;慢性乙型肝炎患者更易重叠CMV感染,并且其感染程度与肝组织的活动性病变密切相关。 Objective To explore the cytomegalovirus(CMV) antigen (CMVAg) expression and distribution in the liver tissue of patients without hepatitis and super infected with hepatitis B virus(HBV) as well as the relationship between CMV and HBV. Methods Liver tissues from 31 non-hepatic disese patients and 76 chronic hepatitis patients, including (44 cases with mild and 32 cases with marked chronic hepatitis) were detected by immunohistochemical staining with monoclonal anti-CMVAg, and 76 cases with chronic hepatitis B virus were studied by double labeling techniques using polyclonal anti-HBc and monoclonal anti-CMV. Results The total positive rate of CMVAg was 22.6%(7/31 cases) in the patients without hepatitis and lower than in the patients with chronic hepatitis B, (56.6%(43/76) cases, P〈0.01). CMVAg was mainly found in the cytoplasm of hepatocytes,CMVAg positive cells were less in the patients without hepatitis than the patients with chronic hepatitis B. The distribution of CMVAg positive cells in the cases with hepatitis B virus might be aggregated near periportal areas or scattered in intralabular area. There was no significant difference of positive rates between mild and marked chronic hepatitis cases( P〉0.05). Double labeling studies showed that both of CMVAg and HBcAg might be expressed mainly in the same area of liver even in same hepatocytes in most cases,but also in different intralabular area. Conclusion CMV infection in the patients without hepatitis is no less seen. Cytomegalovirus is more frequently super infected on the patients with chronic hepatitis B,and the degree of CMV infection is closely associated with liver inflammation.
出处 《临床荟萃》 CAS 2009年第8期666-668,共3页 Clinical Focus
关键词 肝炎 乙型 慢性 巨细胞病毒 免疫组织化学 hepatitis B, chronic cytomegalovirus immunohistochemistry
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