排龈药物对体外培养人牙龈成纤维细胞的毒性比较

Comparison of cytotoxicity between chemical retraction agents on human gingival fibroblasts in vitro

目的评价6种常用的排龈药物对人牙龈成纤维细胞(HGF)毒性作用的大小,以指导临床选择最佳排龈药物。方法将6种不同体积分数的排龈药物(20%硫酸铝、5%硫酸铝、15.5%硫酸铁、13.3%硫酸铁、0.1%盐酸肾上腺素、0.01%盐酸肾上腺素)分别作用于体外培养的HGF,MTT比色法测定细胞的损伤与增殖,透射电镜观察细胞超微结构的变化。结果所有实验药物均能引起细胞的直接损伤与增殖抑制,细胞毒性由小到大分别是0.01%盐酸肾上腺素、0.1%盐酸肾上腺素、5%硫酸铝、20%硫酸铝、硫酸铁,2种体积分数的硫酸铁毒性比较差异无统计学意义(P>0.01)。透射电镜显示2种体积分数的盐酸肾上腺素及5%硫酸铝可引起细胞器数量减少,线粒体及内质网肿胀;20%硫酸铝造成细胞水肿明显,细胞核和染色质分布不均匀;硫酸铁可导致细胞变性。结论0.01%盐酸肾上腺素细胞毒性作用最小,硫酸铁毒性最大。

Objective To choose the best retraction agent for the clinic by evaluating cytotoxic effects of six chemical retraction agents on human gingival fibroblasts （HGF） in vitro. Methods HGF were treated with six chemical retraction agents which included 20% Al2（SO4）3, 5% Al2（SO4）3, 15.5% Fe2（SO4）3, 13.3% Fe2（SO4）3, 0.1% HCl- epinephrine and 0.01% HCl-epinephrine. The damage of direct contact and proliferation inhibit were observed by methyl thiazolyl tetrazolium （MTF） colorimetric assay, the ultra structure was observed under transmission electron microscope （TEM）. Results All of chemical retraction agents caused cell damage and proliferation inhibit. The ability of cytotoxic effect increased in an order of 0.01% HCl-epinephrine〈0.1% HCl-epinephrine〈5% Al2（SO4）3〈 20% Al2（SO4）3〈Fe2（SO4）3. There was no statistically significant difference between 13.3% Fe2（SO4）3 group and 15.5% Fe2 （SO4） 3 group. At HCl-epinephrine group and 5% Al2 （SO4）3 group, the number of organelles were decreased, mitochondria and endoplasmic reticulum were swollen. At 20% Al2 （SO4）3 group, organelles swelling and decreased number of organelles became more severe, the nuclear chromatin was unevenly distributed. Cell degeneration could be made by Fe2 （SO4）3. Conclusion 0.01% HCl-epinephrine was the least toxic gingival retraction agents, 13.3% Fe2（SO4）3 and 15.5% Fe2（SO4）3 are found to have the strongest cytotoxic effect on cell cultures.