头部电针透穴对帕金森病模型大鼠细胞凋亡的作用机制研究

Study on the mechanism of electroacupuncture scalp point penetration therapy in action on apoptosis in the Parkinson's disease rat model

目的:探讨头部电针透穴治疗帕金森病(PD)的作用机制。方法:将40只Wistar大鼠随机分为正常组、假手术组、模型组、针刺组,以6-羟基多巴胺(6-0HDA)左侧纹状体注射法制备偏侧PD大鼠模型,针刺组穴取"百会"透"太阳",每日1次,6日为一疗程。其他3组不予治疗,观察2个疗程。采用免疫组织化学方法观察各组左侧黑质神经营养因子(BDNF)面密度(阳性目标总面积与统计场总面积比值)、积分光密度,用高效液相色谱观察左侧纹状体多巴胺(DA)含量,以TUNEL法观察各组细胞凋亡数。结果:针刺组与模型组比较,左侧黑质BDNF面密度、积分光密度均显著增大(P<0.05);细胞凋亡数量为(23.80±3.83)个,显著减少(P<0.05);纹状体DA含量显著增多(P<0.05)。结论:头部电针透穴可能是通过增强黑质BDNF蛋白表达水平来减少细胞凋亡数量。

Objective To explore the mechanism of electroacupuncture scalp point penetration therapy in treatment of the Parkinson＇s disease （PD）. Methods Forty Wistar rats were randomly divided into a normal group, a sham-operation group, a model group and an electroacupuncture （EA） group. 6-OHDA was injected into the left striatum to make lateralization PD rat model. Acupuncture at ＂Baihui＂ （GV 20）-through-＂Taiyang＂ （EX-HN 5）, once each day, 6 days constituting one course. Immunohistochemical method was used to observe the facio-density and the integral optical density of brain-derived neurotrophic factor （BDNF） in the left substantia nigra, and TUNEL method was used to observe the apoptotic amount, and high performance liquid chromatography was used to observe DA contents of the left striatum in each group. Results As compared with the model group, in the acupuncture group, the facio-density and the integral optical density in the left substantia nigra increased significantly （P〈0. 05）, the amount of apoptosis decreased significantly （P〈0. 05）, and the content of striatum DA increased significantly （P〈0.05）. Conclusion EA scalp point-through-point therapy may enhance BDNF protein expression level in the substantia nigra to decrease the amount of apoptosis in the PD model rat.