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三七对酒精性肝病大鼠肝组织转化生长因子β表达的影响 被引量:2

Effect of Sanchi on Hepatic Expression of TGF-β in Rats with Alcoholic Liver Disease
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摘要 目的:观察三七对酒精性肝病(ALD)大鼠肝组织转化生长因子-β(TGF-β)表达的影响。方法:110只雄性SD大鼠随机分为正常组30只,模型组35只,三七高剂量组、三七低剂量组和硫普罗宁组各15只,连续14周白酒-玉米油-吡唑混合液灌胃建立ALD模型,造模同时三七高、低剂量组和硫普罗宁组分别灌服1.2g/(kg体重.天)、0.6g/(kg体重.天)的三七粉及0.1g(kg体重.天)的硫普罗宁;4周末、8周末、14周末正常组分别随机处死大鼠10只,模型组分别随机处死大鼠8只用于肝脏病理变化的动态观察;14周末处死剩余所有大鼠,光镜观察肝组织脂肪变、炎症及纤维化程度,酶法测定血清ALT、AST水平,脂酶法测定血脂(TG、CHOL、HDL-C、LDL-C),免疫组化二步法检测肝组织TGF-β蛋白的表达。结果:与同期正常组相比,各时间点模型组大鼠肝组织脂肪变及炎症程度计分明显增高(P<0.01);8周末、14周末肝脏TGF-β表达明显增加(P<0.05);实验结束时模型组大鼠血清ALT、AST及CHOL、HDL-C水平较正常组明显增高(P<0.01)。三七高、低剂量组及硫普罗宁组大鼠肝组织脂肪变及炎症程度、血清ALT、AST水平和肝脏TGF-β蛋白表达较模型组明显减轻或降低(P<0.01或0.05),对血脂未见明显影响(P>0.05)。结论:用白酒-玉米油-吡唑混合液灌胃14周能成功复制大鼠ALD模型;三七可明显减轻ALD大鼠肝组织脂肪变和炎症程度,抑制ALD大鼠肝脏TGF-β蛋白的表达,后者可能是三七有效防治ALD的机制之一。 Objective : To evaluate the effect of Sanchi on hepatic expression of transforming growth factor β ( TGF - β ) in rats with alcoholic liver disease (ALD). Methods: 110 SD male rats were derided into normal group ( n = 30), control group( n =35) ,high-dose Sanchi group( n = 15) ,low- dose Sanchi group( n = 15) and Tiopronin group( n = 15) randomly. A formula of ethanolcorn oil- pyrazole mixture was selected to induce ALD model, fed by lavage 14 weeks continually, meanwhile, rats of high - dose Sanchi group, low - dose Sanchi group and Tiopronin group were provided with 1.2g/( kg· d ) Sanchi, 0.6g/( kg ·d) Sanehi, 0.1 g/( kg · d) Tiopronin respectively. For investigating dynamic histologie changes,10 rats in normal group and 8 rats in control group were sacrificed at the end of 4^th week,8^th week,14^th week by turns, and the remnants were sacrificed at the end of 14^th week. Histopathological scores were assessed by microscopie examination of HE and Masson staining of the right lobe of liver. Serum ALT and AST were detected with enzymic method,serum TG, CHOL,HDL- C, LDL- C were detected with lipidase method, hepatic expression of TGFβ were detected with immunohlstochemistry. Results: Compared with the rats in normal group, rats in control group developed higher steatosis scores and inflammation scores at every point( P 〈0.01 ). whose serum ALT,AST and CHOL,HDL- C were obvious higher than the controls at the end of the study( P 〈 0.01 ) ,the similar results were observed about the hepatic expression of TGFβ( P 〈0.05) at the end of 4th week and 14th week . Prevented with high- dose Sanehi,low-dose Sanchi ,or Tiopronin ameliorated the histopathological changes remarkably( P 〈0.01 or 0.05), the serum ALT,AST and expression of hepatic TGFβ were obvious inhibited( P 〈 0.01 or 0. 05 ) ,but the serum CHOL,HDL- C hadn't be affected ( P 〉 0. 05 ). Conclusion: Daily dosing of ethanol - corn oil - pyrazole mixture for 14 weeks can replicate rat ALl) model successfully. Sanchi can remarkable restrain the hepatic expression of TGFβ,then improves the hepatic steatosis and inflammation.
出处 《中华中医药学刊》 CAS 2009年第4期817-821,共5页 Chinese Archives of Traditional Chinese Medicine
基金 杭州市医药卫生科技计划项目(2006B056)
关键词 肝疾病 酒精性 大鼠 三七 转化生长因子-Β liver disease alcoholic rat sanchi TGF - β
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