安胎合剂对GnRHa超排卵小鼠子宫内膜容受性的影响

Effect of Antai Mixture on Endomertial Receptivity of Superovulation Mice by GnRHa

目的:探讨中药安胎合剂对促性腺激素释放激素激动剂(GnRHa)超排卵小鼠子宫内膜容受性影响的作用机理。方法:将45只小鼠随机分成3组:模型组(GnRHa+HMG+HCG)即GnRHa长周期促排卵方案,治疗组(安胎合剂+GnRHa+HMG+HCG),对照组为生理盐水组。采用免疫组化法(SP两步法、Envision法)检测子宫内膜整合素β3、白血病抑制因子(LIF)以及腺上皮细胞雌激素受体(ER)、孕激素受体(PR)的表达。结果:①治疗组子宫内膜整合素β3、白血病抑制因子表达呈强阳性,与模型组比较有显著性差异(P<0.01)。②治疗组子宫内膜腺上皮细胞雌孕激素受体的阳性率明显高于模型组(P<0.001)。结论:中药安胎合剂可能通过促进GnRHa长周期超排卵小鼠子宫内膜整合素β3、白血病抑制因子以及腺上皮细胞雌孕激素受体的表达,以改善其子宫内膜容受性,提高妊娠率。

Objective：To investigate the mechanism of Antai Mixture on Endomertial Receptivity of superovulation mice by GnRHa.Methods：45 Rats were randomly divided into there groups： model group（GnRHa＋HMG＋HCG）,therapeutic group（Antai＋GnRHa＋HMG＋HCG）and control group.The levels of expression of integrinsβ3,leukemia inhibitory factor（LIF）,estrogen receptor（ER） and progestin receptor（PR）in glandular epithelium cells by immunohistochemistry techniques.Results：①The levels of expression of integrinsβ3 were found more stronger in therapeutic group than in model group（P〈0.01）.②For the positive rates of ER and PR in in glandular epithelium cells,there was significant diference between therapeutic group and model group（P〈0.001）.Conclusion：Antai Mixture may promote the Endomertial Receptivity of superovulation mice by increasing the expression of integrinsβ3,leukemia inhibitory factot（LIF）,estrogen receptor（ER）and progestin receptor（PR） in glandular epithelium cells.