聚合酶链反应检测冠心病患者外周血人巨细胞病毒

The detection of cytomegalovirus in the peripheral blood cells of patients with coronary heart disease

目的：探讨人类巨细胞病毒（HCMV）与动脉粥样硬化（AS）的关系及外周血检测的意义。方法：应用聚合酶链反应特异扩增HCMV早期抗原区。结果：冠心病组64例外周血阳性43例（67.2％）；健康对照组30例中阳性3例（10.0％），两组比较，差异有显著意义（P＜0.05）。结论：HCMV可能是AS的一种致病因子，并可从外周血中检出。

Objective:To investigate the relationship between coronary heart disease and cytomegalovirus (CMV) infection. Methods:Major immediate-early antigen region of CMV was distingiushed by using ploymerase chain reaction (PCR). Results:In 64 patients with coronary heart disease,43 cases (67.1 %) were considered as positive for CMV infection. In comparasion,positive band was detected in only 10% (3/30 cases) of healthy objectives. This difference in CMV infection between two groups was statistically significant (P <0.05). Conclusion :CMV infection may be an important factor in the pathogenesis of atherosclerosis and this virus can be detected in the peripheral blood cells.