RP-HPLC法测定不同产地红枣中甜菜碱的含量

Determination of Betaine in Zizyphus Jujube from Different Habitats by RP-HPLC Technique

目的:通过对影响甜菜碱提取和测定的各种因素进行了研究,建立了一种准确、快速的测定不同产地红枣中甜菜碱的方法。方法:样品采用超声波甲醇提取的方法,得到的样品采用反相C18柱进行分离,色谱条件为:流动相为50mmol/L pH4.5的KH2PO4溶液;192nm波长测定,流速0.7ml/min。结果:总分析时间:4.5min。甜菜碱平均保留时间2.698min。甜菜碱在5.0～25.0mg/ml线性关系良好(r2=0.9911),平均回收率为96.87%。结论:该法测定红枣中甜菜碱含量简便、快速、准确,重现性好。

Objective：A series of studies was conducted to establish a methodology for the accurate and efficient determination of betaine in Zizyphus. Jujube from different habitats. Method：The final methodology involves an extraction step in which the sample is extracted with ultrasonic. An HPLC method using an ODS-C18 column and UV detection for the determination of betaine in plant tissue extrats is described. The mobile phase is kept constant at pH 4.5 and 50mmol/L KH2PO4 in water, and eluted compounds are detected by UV absorbance （192nm）. The flow rate is mained at 0.7ml/min. Result：Retention time for the betaine was 2.698min and a single run totally with the betaine 4.5 minutes only. The calibration curve was linear over the range of 5.0～25.0 mg/ml （r2=0.9911）.The average recovery was 96.87%. Conclusion：This method is simple, reliable, repeatable, and is suitable for the determination of betaine in Zizyphus. Jujube.