摘要
目的:研究牙周病患牙累及的牙根面经EDTA脱矿和富血小板血浆(PRP)单独及联合处理后对人牙周膜成纤维细胞附着、增殖的影响。方法:以因牙周病不能保留而拔除的患牙制备的牙根片为对照组,PRP和EDTA脱矿单独及联合处理患牙根片为实验组,用细胞计数法及四甲基偶氮唑盐(MTT)比色实验分析人牙周膜成纤维细胞在病变根面上的附着和增殖。结果:未处理组的根片上有少量的细胞附着;根片经PRP或EDTA脱矿单独处理能明显增加细胞的附着(P<0.05);二者联合处理促附着效果明显增强(P<0.01)。未处理组根片上生长的细胞随时间延长数量反而下降;经PRP或EDTA单独处理的根片上的细胞有少量的增殖(P>0.05);二者联合处理细胞增殖明显(P<0.05)。结论:EDTA根面脱矿和PRP能有效促进人牙周膜成纤维细胞在牙周病患牙根面上的附着和增殖。
Objective: To evaluate the effect of EDTA and platelet-rich plasma (PRP) on the attachment and proliferation of human periodontal ligament fibroblasts on diseased root surfaces in vitro. Methods: Periodontitis-affected teeth were collected and sectioned with a water-cooled diamond disc to prepare the 4mm × 4mm diseased root slices. The diseased root surfaces slices were then scaled . Test slices were exposured to either EDTA (24% , pH = 6.7) or PRP. The controls were treated with nothing. Double-sided adhesive tape was used to fix the root slices to the bottom of wells in a 48-well tissue culture plate. The treated and untreated root slices were then sterilized under UV light for 2 hours prior to seeding with cells . The culture were allowed to attach for 24 or 48 hours using standard incubation conditions. The attached and proliferated cells were visualized using MTT staining. Results: The untreated root surfaces demonstrated the least number of attached cells. The root surfaces treated with EDTA or PRP alone all significantly increased cell attachment( P 〈 0.05 ). When PRP were added to previously EDTA-etched root surfaces, cell attachment was further enhanced (P 〈0.01 ). Diseased root surfaces untreated with EDTA or PRP did not enhance cell proliferation, cell proliferation onto diseased root surfaces treated with EDTA or PRP alone was slightly increased (P 〉 0.05). When PRP were added to previously EDTA-etehed root surfaces, cell proliferation was also enhanced. ( P 〈 0.05). Conclusion: Freshly prepared PRP or EDTA could effectively enhance the attachment and proliferation of human periodontal ligament fibroblasts onto human diseased root surfaces.
出处
《海南医学院学报》
CAS
2009年第5期437-439,共3页
Journal of Hainan Medical University
基金
海南医学院科研基金资助项目(0020091)
关键词
富血小板血浆
牙周膜成纤维细胞
附着
增殖
Platelet-rich plasma
Periodontal ligament fibroblasts
Attachment
Proliferation