期刊文献+

牙周病患牙根面的不同处理对牙周膜成纤维细胞生长的影响 被引量:1

Effects of deminerazation and platelet-rich plasma on periodontal ligament fibroblasts attachment and proliferation on diseased root surfaces
下载PDF
导出
摘要 目的:研究牙周病患牙累及的牙根面经EDTA脱矿和富血小板血浆(PRP)单独及联合处理后对人牙周膜成纤维细胞附着、增殖的影响。方法:以因牙周病不能保留而拔除的患牙制备的牙根片为对照组,PRP和EDTA脱矿单独及联合处理患牙根片为实验组,用细胞计数法及四甲基偶氮唑盐(MTT)比色实验分析人牙周膜成纤维细胞在病变根面上的附着和增殖。结果:未处理组的根片上有少量的细胞附着;根片经PRP或EDTA脱矿单独处理能明显增加细胞的附着(P<0.05);二者联合处理促附着效果明显增强(P<0.01)。未处理组根片上生长的细胞随时间延长数量反而下降;经PRP或EDTA单独处理的根片上的细胞有少量的增殖(P>0.05);二者联合处理细胞增殖明显(P<0.05)。结论:EDTA根面脱矿和PRP能有效促进人牙周膜成纤维细胞在牙周病患牙根面上的附着和增殖。 Objective: To evaluate the effect of EDTA and platelet-rich plasma (PRP) on the attachment and proliferation of human periodontal ligament fibroblasts on diseased root surfaces in vitro. Methods: Periodontitis-affected teeth were collected and sectioned with a water-cooled diamond disc to prepare the 4mm × 4mm diseased root slices. The diseased root surfaces slices were then scaled . Test slices were exposured to either EDTA (24% , pH = 6.7) or PRP. The controls were treated with nothing. Double-sided adhesive tape was used to fix the root slices to the bottom of wells in a 48-well tissue culture plate. The treated and untreated root slices were then sterilized under UV light for 2 hours prior to seeding with cells . The culture were allowed to attach for 24 or 48 hours using standard incubation conditions. The attached and proliferated cells were visualized using MTT staining. Results: The untreated root surfaces demonstrated the least number of attached cells. The root surfaces treated with EDTA or PRP alone all significantly increased cell attachment( P 〈 0.05 ). When PRP were added to previously EDTA-etched root surfaces, cell attachment was further enhanced (P 〈0.01 ). Diseased root surfaces untreated with EDTA or PRP did not enhance cell proliferation, cell proliferation onto diseased root surfaces treated with EDTA or PRP alone was slightly increased (P 〉 0.05). When PRP were added to previously EDTA-etehed root surfaces, cell proliferation was also enhanced. ( P 〈 0.05). Conclusion: Freshly prepared PRP or EDTA could effectively enhance the attachment and proliferation of human periodontal ligament fibroblasts onto human diseased root surfaces.
出处 《海南医学院学报》 CAS 2009年第5期437-439,共3页 Journal of Hainan Medical University
基金 海南医学院科研基金资助项目(0020091)
关键词 富血小板血浆 牙周膜成纤维细胞 附着 增殖 Platelet-rich plasma Periodontal ligament fibroblasts Attachment Proliferation
  • 引文网络
  • 相关文献

参考文献6

  • 1Okuda K , Kanase T , Momose M , et al . Platelet-rich plasma contains high levels of platelet-derived growth factor and transforming growth factor-beta and modulates the proliferation of periodontally related cells in vitro[ J ] . J Periodontal,2003 , 74 (6) : 849-857 .
  • 2Lekovic V , Camargo PM , Weinlaender M , et al . Comparison of platelet-rich plasma , bovine porous bone mineral, and guided tissue regeneration versusplatelet-rieh plasma and bovine porous bone mineral in the treatment of intrabony defects : a reentry study [ J ]. J Periodontol,2002, 73(2) :198-205 .
  • 3刘琪,Victor Marino,Mark Bartold.新型牙周再生细胞传递载体的体外建立[J].华西口腔医学杂志,2006,24(1):15-17. 被引量:8
  • 4Gonshor A. Technique for producing platelet-rich plasma and platelet concentrate: background and process [ J ]. Int J periodontics Restorative Dent, 2002,22 ( 6 ) :547-557.
  • 5Sculean A, Donos N, Brecx M, et al. Treatment of intrabony defects with guided tissue regeneration and enamelmatrix-proteins. An experimental study in monkeys [ J ]. J Clin Periodontol,2000 ,27 ( 7 ) :466-72.
  • 6Kawase T, Okuda K, Wolff LF, et al. Platelet-rich plasma-derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoblastic cells in vitro [J]. J Periodontol,2003,74(6):858-64.

二级参考文献17

  • 1Bouvier M,Couble ML,Hartmann DJ,et al.Isolation and characterization of rat alveolar bone cells[J].Cell Mol Biol,1991,37(5):509-517.
  • 2Gonshor A.Technique for producing platelet-rich plasma and platelet concentrate:Background and process[J].Int J Periodontics Res Dent,2002,22(6):547-557.
  • 3Hillmann G,Steinkamp-Zucht A,Geurtsen W,et al.Culture of primary human gingival fibroblasts on biodegradable membranes[J].Biomaterials,2002,23(6):1461-1469.
  • 4Farrell DH,al-Mondhiry HA.Human fibroblast adhesion to fibrinogen[J].Biochemistry,1997,36(5):1123-1128.
  • 5Salonen JI,Persson GR.Migration of epithelial cells on materials used in guided tissue regeneration[J].J Periodontal Res,1990,25(4):215-221.
  • 6Simain-Sato F,Lahmouzi J,Kalykakis GK,et al.Culture of gingival fibroblasts on bioabsorbable regenerative materials in vitro[J].J Periodontol,1999,70(10):1234-1239.
  • 7Hammerle CHF.Membranes and bone substitutes in guided bone regeneration[C].Proceedings of the 3rd European workshop on Periodontology,1999,3:468-499.
  • 8Hughes FJ,Aubin JE,Heersche JN.Differential chemotactic responses of different population of fetal rat calvaria cells to platelet-derived growth factor and transforming growth factorβ[J].Bone Miner,1992,19(1):63-74.
  • 9Bruder SP,Fox BS.Tissue engineering of bone:Cell-based strategies[J].Clin Orthop Relat Res,1999,(367 Suppl):S68-S83.
  • 10Takata T,Wang HL,Miyauchi M.Attachment,proliferation and differentiation of periodontal ligament cells on various guided tissue regeneration membranes[J].J Periodontal Res,2001,36 (5):322-327.

共引文献7

同被引文献12

引证文献1

二级引证文献3

;
使用帮助 返回顶部