摘要
目的建立大鼠酒精性肝病模型。方法应用逐渐增加酒精浓度(30%~60%)和剂量(5~9 g/kg·d^(-1))的方法.每日给予大鼠灌胃2次.分别于0、4、8、12、16周末留取血清和肝组织标本,检测血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、胆碱酯酶、氧化应激指标和肝组织病理变化。结果随着病程进展,出现肝功能受损、氧化应激指标变化,肝组织病理呈现肝细胞脂肪变、小叶内坏死灶和纤维化改变。结论逐渐增加浓度和剂量的酒精直接灌胃法可建立大鼠慢性酒精性肝病模型。
Objective To establish an alcoholic liver disease model in rats. Methods Wistar rats were lavaged by alcohol of increasing concentration and dose gradually(30%-60%, 5-9 g/ kg^-1 · d^-1) and the alcohol was given twice a day. To perform a time-course study, rats were sacrificed randomly at the end of 4th, 8th, 12th and 16th week and the serum, liver homogenate (10%) and liver samples were collected respectively. Results With the progress of disease, hepatic cells steatosis,necrosis and fibrosis in liver lobes,were observed in hepatic tissue,and the level of aspartate aminotransferase and alanine aminotransferase in serum, triglyceride, oxygen free radical,malonaldehyde and thromboxane B2 in liver homogenate of rats increased significantly while the cholinesterase in the serum and content of superoxide dismutase and antisuperoxide anion in liver homogenate decreased. Conclusion Experimental model of chronic alcoholic liver injury can be established in rats with directly intragastric administration.
出处
《河北医科大学学报》
CAS
2009年第4期328-330,433,共4页
Journal of Hebei Medical University
关键词
肝疾病
酒精
模型
动物
大鼠
liver diseases, alcoholic
models, animal
rats