食管鳞癌中人乳头瘤病毒16型E6、E7和细胞特异性增强子片段的检测

Detection of E6,E7 and cell-type specific enhancer of human papillomavirus type 16 in esophageal squamous cell carcinoma

目的:检测食管鳞癌组织中人乳头瘤病毒(HPV)16E6、E7基因和细胞特异性增强子片段(CTSE)。方法:采用聚合酶链反应法(PCR)检测40例食管鳞癌和20例正常食管黏膜中HPV16E6、E7基因和病毒长控制区内(long control region,LCR)的细胞型特异性增强子(cell type specific enhancer,CTSE)。结果:在40例食管鳞癌中,HPV16的E6、E7基因和CTSE片段的检出率分别是37.5%(15/40)、42.5%(17/40)和40%(16/40),20例正常食管黏膜中E6、E7和CTSE的检出率分别为0%(0/20)、0%(0/20)和5%(1/20),两者均存在显著性差异(P<0.05)。CTSE片段分别与E6和E7基因有明显相关性(P<0.05)。食管鳞癌中E6、E7基因及CTSE的检出率在患者不同性别、年龄、肿瘤浸润程度、淋巴结转移和组织学分级肿瘤中差异均无显著性(P>0.05)。结论:E6和E7基因与CTSE片段共存于HPV16感染的食管鳞癌组织中,三者可能与食管鳞癌的发生和发展有关。

Objective：To detect HPV16 E6, E7 gene and cell -type specific enhancer （CTSE） of long control region （LCR） in esophageal squamous cell carcinoma （ESCC）. Methods：E6, E7 genes and CTSE of HPVI6 were detected in 40 ESCCs and 20 normal esophageal musoca（ NEMs ） through polymerase chain reaction（PCR）. Results： The positive rates of HPV16 E6 gene ,E7 gene and CTSE were 37.5% （15/40） ,42.5% （17/40） and 40% （16/40） respectively in ESCCs but 0% （0/20） ,0% （0/20） and 5% （1/20） in NEMs,which indicated that the positive rates of target genes in ESCCs were statistically higher than those in NEMs respectively （ P 〈 0.05 ）. There exited significant correlation between E6 gene and CTSE in ESCCs （ P 〈 0.05 ） so did between E7 gene and CTSE. HPV16 E6 gene had no statistical correlation with gender, age,invasion, histology grades and lymph nodes metastasis repectively （P 〉0.05） ,nor did E7 gene and CTSE（P 〉0.05）. Conclusion：That there existed E6 gene,E7 gene and CTSE together in ESCCs infected by HPV16 and those might have correlation with occurrence and development of ESCC.