摘要
目的:探讨腹腔注射单唾液酸四己糖神经节苷脂(GM1)对幼鼠癫痫持续状态(SE)所致海马损伤是否具有保护作用。方法:18日龄SD大鼠48只随机分成GM1治疗组、SE模型组、正常对照组。经腹腔注射氯化锂-匹罗卡品诱发60min的SE发作,观察大鼠海马组织的病理改变;利用Morris水迷宫实验评价大鼠的学习和记忆能力。结果:GM1治疗组大鼠海马组织的病理变化比SE模型组轻,其海马CA1区的神经元凋亡和丢失数量明显减少。Morris水迷宫实验结果提示:与SE模型组比较,GM1治疗组的平均寻台潜伏期明显缩短,而在平台区的搜索时间和穿越平台区的次数明显增多。结论:幼年大鼠SE后给予大剂量GM1治疗,可抑制大鼠海马区的神经元的凋亡和丢失,有效地改善大鼠远期的学习记忆功能。
Objective：To investigate the effect of monosialoganglioside （GM1） on Hippocampal injury induced by status epilepticus in juvenile rats. Methods： Forty-eight SD juvenile rats were divided randomly into GM1 treated group,SE and normal control group. Status epilepticus （over 60 minutes）was induced in juvenile rats by intraperitoneal injection with lithium-pilocarpine. The pathological changes in Hippoeampal tissues were observed. Morris water maze was used to evaluate the learning and memory of rats. Results ：Histopathological evaluation demonstrated that GM1 significantly diminished Hippocampal injury and decreased the cell apoptosis in Hippocampal CA1 region. In GM1 treated group, the mean latencies to reach the platform was significantly shorter and the time spent in the target quadrant was significantly longer than those in control group （P〈0.05）. Conclusion： The GM1 can inhibit the apoptosis of neural cells in Hippocampal CA1 region after status epilepticus, and significantly improve the ability of learning and memory.
出处
《陕西医学杂志》
CAS
2009年第5期529-531,543,共4页
Shaanxi Medical Journal
