哈维氏弧菌和溶藻弧菌对大黄鱼6种酶活性的影响

Effects of Vibrio harveyi and V.anguillarum on Enzymes Activities in Different Organs of Large Yellow Croaker(Pseudosciaena crocea)

以哈维氏弧菌(Vibrio harveyi)和溶藻弧菌(Vibrio anguilarum)分别对大黄鱼(Pseudosciaena crocea)进行病原接种实验,24 h后测定肝、脾、鳃的碱性磷酸酶(Alkaline phosphatase,AKP)、酸性磷酸酶(Acid phosphatase,ACP)、过氧化氢酶(Catalase,CAT)、超氧化物歧化酶(Superoxide dismutase,SOD)、溶菌酶(Lysozyme,LSZ)和酚氧化酶(Phenoloxi-dase,PO)活性.结果表明:弧菌入侵24 h后,与吞噬作用相关的水解酶ACP、AKP变化不显著,肝脏溶菌酶LSZ活性降低,出现典型病症,可能是由于应激反应引起非特异性免疫机能下降所致;抗氧化酶CAT、SOD变化显著,对清除代谢产生的活性氧有重要作用;6种酶中,LSZ、CAT、SOD和PO对病原反应敏感,适合作为2种弧菌入侵的检测指标.

The activities of alkaline phosphatase（AKP）, acid phosphatase（ACP）, eatalase（CAT ）, superoxide dismutase（SOD）, lysozyme（LSZ） ,and phenoloxidase（PO） were investigated in different organs at 24th hour after the Pseudosciaena crocea was injected with Vibrio harveyi ,Vibrio anguillarum and normal saline（control group） respectively. The result showed that,except the activities of ACP from the group injected with Vibrio anguillarum were higher than those in control group,the activities of ACP and ALP in the other groups were not significantly different from the control. Meanwhile, the activities of LSZ in liver were lower than that of the control observably,maybe due to the non-specific immunity was restrained by stress reactions. In conclusion, the activities of LSZ, CAT,SOD and PO changed significantly,in terms of that they could be the indicators of vibrios infection.