染料木黄酮对哮喘豚鼠肺部炎症和气道重塑的预防作用

THE PREVENTATIVE EFFECTS OF PROTEIN TYROSINE KINASE ON THE INFLAMMATION AND AIRWAY REMONDELING IN LUNG OF GUINEA PIGS WITH BRONCHIAL ASTHMA

目的:研究蛋白酪氨酸激酶(PTK)抑制剂染料木黄酮对哮喘豚鼠肺部炎症和气道重塑的作用。方法:成年雄性豚鼠30只,随机分成3组(n=10):对照组(C组)、哮喘组(A组)和染料木黄酮干预组(B组),以腹腔内注射联合雾化吸入卵蛋白复制哮喘模型。测支气管肺泡灌洗液(BALF)中细胞总数及其分类数,测细支气管炎症细胞浸润及支气管重塑指标,免疫组化方法测磷酸化酪氨酸(p-tyrosine)在肺组织中的表达。结果:A组BALF中细胞总数、嗜酸性粒细胞分类与C组比较明显增加,B组与A组比较明显降低,差异有统计学意义(P<0.01);A组细支气管嗜酸性粒细胞(E)数和淋巴细胞(L)数较C组明显增多,B组与A组比较明显降低,差异有统计学意义(P<0.01);B组细支气管重塑较A组明显减轻(P<0.01),与C组比较,差异无统计学意义(P<0.05);免疫组化显示p-tyrosine在支气管平滑肌、支气管上皮、血管滑平滑肌及炎性细胞均有表达,尤其以支气管和血管平滑肌及炎性细胞明显,A组比C组表达明显增高,差异有统计学意义(P<0.01),而B组与C组比较,无明显差别(P>0.05)。结论:PTK对哮喘豚鼠肺部炎症和支气管重塑具有促进作用:PTK抑制剂染料木黄酮对哮喘豚鼠肺炎症和支气管重塑具有预防和抑制作用。

Aim： To investigate the effects of protein tyrosine kinase on the inflammation and airway remodeling in lung of guinea pigs with bronchial asthma. Methods： 30 adult male guinea pigs were randomly divided into 3 groups（ n = 3）： control group（ C group）, asthmatic group（A group）and genistein group（B group）. Asthmatic model was established by ovalbumin intraperitoneal injection and ovalbumin inhalation. The total cell and the proportion of inflammatory cell in bronchial alveolar lavage fluid（ BALF）, inflammatory cell infiltration and index of remodeling of bronchiole were measured, respectively. The expression of p-tyrosine in lung tissue was examined by immunohistochemistry. Results： The total cell and proportion of eosinophll in BALF of A group were significantly higher than that of C group（ P 〈 0.01 ）, but compared with A group, the total cell and proportion of eosinophil in BALF of B group were much lower（ P 〈 0.01 ）. The number of eosinophile and lymphocyte of bronchiole in A group were significantly higher than that of C group（ P 〈 0.01 ）, but compared with A group, the number of eosinophile and lymphocyte in bronchiole of B group were much lower（ P 〈 0. 01 ）. Compared with A group, the remodeling of bronchiole of B group was significautly relieved（ P 〈 0.01 ）, there was no difference between B and C group（ P 〉 0.05）. Immunohistochemistry indicated that in A group the p-tymsine was more positively expressed at the bronchial smooth muscle, bronchial epithelium, smooth muscle of vessel and inflammatory cell, especially at smooth muscle of bronchi and vessel and inflammatory cell than that of C group（ P 〈 0.01 ）, there was no difference between B group and C group（ P 〉 0.05）. Conclusion： PTK played a key role in inflammation and bronchial remodehng in lung of guinea pigs with bronchial asthma. The Protein tyrosine kinase inhibitor genistein could prevent and inhibit the inflammation and bronchial remodeling in lung of guinea pigs with bronchial asthma.