摘要
对Arthrobacter chlorophenolicus GS0202菌产人参皂苷糖苷酶进行分离纯化并对其性质进行了研究。结果表明,电泳纯人参皂苷糖苷酶的分子质量约为66ku,最适反应pH值为5.0,最适温度为40℃,在pH3.0～7.0,20～60℃酶活力相对稳定。K+、Na+、Zn2+对酶活性影响很小;Ca2+、Mg2+、Cu2+对酶活性有明显的抑制作用。Km值为4.392mmol/L,Vmax值为0.1275mmol/(h.L)。
The paper was aimed to investigate panaxsaponin-glucosidase which was purified from the broth of microbion （Arthrobacter chlorophenolicus GS0202 ） and its properties. The results showed that the molecular weight of which was 66ku,the optimum pH and temperature were pH 5.0 and 40℃ respectively,the enzyme was stable in the pH range from 3.0 to 7.0 and the temperature ranges from 20℃to 60℃. K^＋ , Na^＋ and Zn^2＋ had little contribution to the enzyme; Ca^2＋ , Mg^2＋ and Cu^2＋ showed noticeable inhibiting attributes. The kinetic analysis revealed that the value of Km and VmAx was 4. 392mmol/L and 0. 1275mmol/（h · L） , respectively.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2009年第4期16-19,共4页
Food and Fermentation Industries
基金
国家自然科学基金资助项目(20476017)
辽宁省高等学校优秀人才支持计划资助项目(RC-04-05)
辽宁省科学技术基金项目(20042132)
