HPLC-ELSD法同时测定盾叶薯蓣根中3种皂苷的含量

HPLC-ELSD simultaneous determination of three components in root of Dioscorea zingiberensis C.H.Wright

目的:建立同时测定盾叶薯蓣根中黄姜素A、盾叶新苷及薯蓣皂苷3种成分含量的HPLC-ELSD分析方法,考察不同产地盾叶薯蓣根中3种成分的含量。方法:药材粉末经70%乙醇回流提取,以HPLC-ELSD法进行测定。色谱柱采用Diamosil C18(250mm×4.6mm,5μm),流动相为甲醇(A)-水(B),梯度洗脱(0～10min,78%A;10～13min,78%A→90%A;13～45min,90%A),流速为1.0mL·min-1,漂移管温度为79.0℃,气体流速为2.1L·min-1。结果:黄姜素A、盾叶新苷和薯蓣皂苷三者均有良好的线性关系;其平均回收率(n=6)分别为100.4%,103.0%,95.3%。结论:本方法操作简便,结果可靠,可用于同时定量分析盾叶薯蓣根中黄姜素A、盾叶新苷及薯蓣皂苷3种成分。

Objective：To establish a quantitative method for the simultaneous determination of Huangjiangsu A, zingiberensis newsaponin and dioscin, and contrast the contents of three compounds in root of Dioscorea zingiberensis C. H. Wright from different habitats. Method：After refluxed with 70% ethanol, samples were separated by Diamonsil C18 column（250 mm×4.6 ram, 5 μm）;The mobile phase consisted of methanol（A） -water（B） with gradient elution（0- 10 rain, 78% A;10- 13 min, 78% A→90% A;13 -45 min, 90% A）,and the flow rate was 1.0 mL·min^-1. The temperature of drift tube and gas flow rate were 79.0℃ and 2.1 L·min^-1, respectively. Results：Huangjiangsu A, zingiberensis newsaponin and dioscin showed good linearity, and the average recoveries were 100. 4% , 103.0% and 95.3% , respectively. Conclusion： The method is simple and accurate for the simultaneous analysis of Huangjiangsu A, zingiberensis newsaponin and dioscin in root of Dioscorea zingiberensis.