卷丹百合种间杂交亲和性研究

Study on Interspecific Hybridization Compatibility of Lilium lancifolium

采用4种授粉方法对卷丹与9种百合进行正反交,研究卷丹的种间杂交亲和性,并对部分杂交组合的花粉管行为进行荧光观察。卷丹作母本的9个组合中有7个组合得到膨大的果实,6个组合得到有胚种子,有胚率最高的为卷丹×大花卷丹,达44.7%。切割花柱可以克服卷丹与有斑百合、大花百合、垂花百合的杂交障碍;涂抹花粉培养液对杂交亲和性影响不大。卷丹作父本与9种百合杂交均不亲和,但有部分组合能得到微膨大的子房,微膨大率可达50%。百合杂交亲和组合的花粉管在48～72 h即可到达花柱基部,72～96 h进入子房与胚珠结合。

Four pollination methods were used to study the cross-compatibility of Lilium lancifolium Thunb. with nine Lilium species including six wild species and three cultivated species. The pollen tube behaviors in some cross combinations were also observed by means of fluorescence microscopy. Fruits were obtained from seven of the nine combinations which L. lancifolium Thunb. was used as the female parent and embryos from six of the nine combinations. The percentage of seeds with embryo of L. lancifolium × L. i reached 44. 7% which was the highest. Cut-style pollination could overcome species-incompatibility between L. lancifolium Thunb. and L. concolor var. buschianum Baker,L. concolor var. megalanthum Wang et Tang or L. cernuum Kom. , while pollination after pollen culture solution had no effects on compatibility of interspecific hybridizatiorL L. lancifolium Thunb. , as the male parent, crossing with the other nine species were incompatible. But some of the combinations got 50% little-swelled fruits. When pollinated after 48-72 h,the pollen tube of compatible combinations reached basal part of the style and finally arrived at the ovule in ovary when pollinated after 72-96 h.