反相高效液相色谱法测定保健食品中阿魏酸的含量

In Opposition Highly Effective Liquid Phase Chromatography Determination Health Foods Ferulic Acid Content

目的建立保健食品中阿魏酸的反相高效液相色谱法测定方法。方法采用反相高效液相色谱法,色谱柱:AichromBond-1 C184.6 mm×150 mm,5μm,流动相:乙睛∶0.085%磷酸水溶液=20∶80检测波长320 nm,流速1.0ml/min,柱箱温度30℃。结果阿魏酸在1.0～10.0μg/ml范围内呈好的线性关系(r=0.999 8),平均回收率为90.3%～94.2%,相对标准偏差分别为6.00%～7.51%。结论该方法简便,准确,能够满足保健食品中阿魏酸含量的检测要求。

Objective To establish the method of ferulic acid in health foods with reversed phase high performance liquid chromatography. Method The reversed phase high performance liquid chromatography was composed. Chromatographic column： Aiehrom Bond-1 C18 4.6 mm ×150 mm, 5 /an. The mobile phase consisted of acetonitril and 0.085% phostrate acid （20：80） ; the wave length was 320 nm; speeds of flow were 1.0 ml/min; column box temperature was 30℃ . Results The ferulic acid appeared the good linear relationship from 1.0-10.0 μg/ml （r = 0.999 8）. The average return-ratio is 90.3%-94.2% . The relatively standard deviations respectively is 6.00%-7.51% . Conclusion This method is simple, accurate, can satisfy the health foods the asafoetida acid content examination request.