摘要
用Protamex蛋白酶,在pH6.5,温度50℃,酶解6h,制得海蜇降压肽。分别以L-NNA致高血压模型和肾动脉狭窄高血压模型的新西兰大白兔为实验对象,灌胃海蜇降压活性肽,研究降压肽的降压效果。结果表明,对两种模型灌胃,均在0.5h左右开始发挥降压作用,剂量0~60mg/kgbw时,降压效果与灌胃剂量呈正相关;灌胃剂量在60mg/kgbw时,降压幅度达到最大,增大灌胃剂量,压降维持时间延长,血压维持正常水平,不会再继续降至正常值以下。对肾动脉狭窄高血压模型动物灌胃相同剂量的情况下,海蜇降血压肽的降压效果优于市售降压药缬沙坦和盐酸贝那普利。
Angiotensin-converting enzyme inhibitory peptides (ACEIP) from jellyfish were prepared via enzymolysis with protease Protamex at pH 6.5 and 50 ℃ for 6 h. Then the anti-hypertensive effects of the ACEIP on narrowed kidney artery caused hypertension and NG-nitro-L-arginine (L-NNA) induced hypertension models in New Zealand white rabbits were studied. The results showed that after 0.5 h of treatment by gavage, the jellyfish ACEIP began to play the antihypertensive effects on both hypertension models. When the dosage was less than 60 mg/kg bw, the anti-hypertensive effect was positively correlative with the dosage. When the dosage was 60 mg/kg bw, the anti-hypertensive effects reached the top, and the increased dosage may not lower the blood pressure below the normal value, but result in longer duration of lowered blood pressure. Compared with commercial anti-hypertensive drugs such as valsartan and benazepril hydrochloride, the jellyfish ACEIP showed the better anti-hypertensive effects against narrowed kidney artery caused hypertension model at the same dosage.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第11期227-231,共5页
Food Science
关键词
高血压
降压肽
制备工艺
高血压模型
hypertension
angiotensin-converting enzyme inhibitory peptides (ACEIP)
technology
hypertension model