Tca8113细胞系下颌下淋巴结转移动物模型的建立

Nude Mice Metastasis Model of Human Oral Squamous Carcinoma Constructed Using Tca8113 Cell

目的:建立人口腔鳞癌Tca8113裸鼠舌体及颊黏膜移植瘤淋巴结转移模型,研究分析两种转移模型的特性。方法:通过在裸鼠舌体及颊黏膜内注射接种Tca8113细胞,建立移植瘤动物模型。舌体及颊部原发灶分别在2周和4周时被切除,观察6周时下颌下淋巴结转移情况。通过组织切片HE染色法计算淋巴结转移率,并采用CK蛋白免疫组织化学染色方法计算淋巴结微灶转移率。结果:13只舌体接种移植瘤裸鼠中,有3只出现下颌下淋巴结转移,5只出现淋巴结微灶转移,转移率达到61.54%。15只颊黏膜接种移植瘤裸鼠中,3只出现下颌下淋巴结转移,3只出现淋巴结微灶转移,转移率为40.00%。结论:裸鼠舌体及颊黏膜接种Tca8113建立的转移模型具有一定的转移率,并能较好地模拟临床口腔鳞癌的转移,为实验研究提供了良好的动物模型。

Objective： To establish two metastasis nude mice models from different cancer locations with Tca8113 cell and clarify their metastasis characters. Methods： Transplantation tumor models of nude mice were established by injecting TcaS113 cells into tongue or buccal mucosa. Transplantation tumors of tongues were removed after 2 weeks and those on buccal mucosa were removed after 4 weeks. 6 weeks later, submandibular lymph nodes were examinated under microscopy. Immunohistoehemistry examination was applied to detect micrometastasis by CK protein. Results： 3 of 13 nude mice with tongue tumors were found metastases in submandibluar lymph nodes, and other 5 mice had lymph node micrometastases. 3 of 15 nude mice with buccal tumors were found metastases in submandibluar lymph nodes and other 3 mice had lymph node micrometastases. The total metastasis rate of rouge tumor model was 61.54%, while those of buccal cancer were 40%. Conclusion： Lymph node metastasis nude mice models can simulate the clinical process of oral squamous cell carcinoma, and show potentials for metastasis research.