摘要
目的建立以高效液相色谱法(HPLC)同时测定枳实中3种黄酮类成分含量的方法。方法色谱柱为Waters XBridge C18柱(4.6mm×150mm,5μm),流动相为乙腈-甲醇-0.2%磷酸溶液(18∶1∶81,v/v/v),流速为1.0ml/min,检测波长为283nm,柱温为室温。结果柚皮苷、橙皮苷和新橙皮苷的浓度分别在0.6000~14.40μg/ml、0.2180~5.232μg/ml和0.6900~16.56μg/ml范围内与峰面积呈良好的线性关系,相关系数分别为0.9995、0.9996和0.9996;平均回收率分别为101.1%、100.8%和100.5%,相对标准偏差值(RSD)分别为1.0%、0.9%和0.7%。结论本方法简便、快捷,结果准确,重复性好,可作为枳实的质量控制方法之一。
Objective To establish an HPLC method for the content determination of three flavonoids in Fructus AurantiiImmaturus. Methods The analytical method was carried out with XBridge C18 (4. 6 mm × 150 mm, 5 um) columnand the mobile phase consisted of acetonitrile- methanol- 0. 2% phosphoric acid solution( 18:1:81 ,v/v/v) as a flow rate of 1.0 ml/min. The detection wavelength was 283 nm. Results The calibration curve was linear within the range of 0. 6000 -14. 40 μg/ml(r = 0.9995),0.2180 -5.232 μg/ml(r = 0.9996) and 0.6900 -16.56 μg/ml (r = 0.9996) for naringin,hesperidin and neohesperidin, respectively. The average reeoverieswere 101.1% (RSD = 1.0 % ) ,100. 8 % ( RSD = 0. 9 % ) and 100. 5 % (RSD = 0. 7 % ) for naringin, hesperidin and neohesperidin, respectively. Conclusion The method is simple, accurate, reproducible and precise, and applicable for the determination of three flavonoids in Fructus Aurantii Immaturus.
出处
《今日药学》
CAS
2009年第6期46-48,共3页
Pharmacy Today