斑马鱼血管紧张素转换酶(ACE)的生物信息学分析

Bioinformatic analysis of angiotensin converting enzyme in Danio rerio

采用生物信息学方法对模式生物斑马鱼中的血管紧张素转换酶进行了系列预测分析。结果表明,斑马鱼中有两个ACE基因,它们所编码的蛋白分别由1 324和785个氨基酸组成,其中1个是酸性蛋白,在水中不稳定,没有信号肽,属线粒体跨膜蛋白;另一个为酸性水溶性稳定蛋白,有信号肽。它们均含保守的Pfam Pep-tidase_M2结构域,属于M2家族蛋白。通过ESTs搜索发现这两个基因在斑马鱼不同组织和不同发育期的表达情况存在较大的差异。同源性比对和系统发育分析表明,斑马鱼ACE蛋白与低等脊椎动物(红原鸡、热带爪蟾等)、高等脊椎动物(人、鼠等)的同源性较高,达60%以上,说明ACE蛋白在系统演化上比较保守。

Angiotensin converting enzyme （ACE） in Danio rerio was predicted and analyzed by using bioinformatic tools. The results showed that there were two ACE genes, i.e. , wu ： fb81h03 and danio ： ace2, encoding 1324 and 782 amino acids, respectively, in Danio rerio genome. Wu ： fb81h03 is an acidic, water-soluble, unstable mito- chondria trans-membrane protein containing no signal peptide. On the other hand, danio ： ace2 is an acidic, watersoluble, stable and contains a signal peptide. Both of them include a conserved domain, Pfam Peptidase_ M2, indicating that they belong to M2 family proteins. Through the analysis of ESTs, these 2 genes were found to express differentially in different tissues and growth stages. Homology BLAST and phylogenetic analysis indicated that D. rerio ACE was close to ACEs from lower vertebrates （e. g. , Gallus gallus and Xenopus （Silurana） tropicalis） and higher vertebrates （e. g. , human and mouse）. The homological percentage was more than 60%, suggesting that the ACE was conserved through the evolution process.