滇重楼皂苷部位HPLC指纹图谱的研究

Study on HPLC-FPS of Saponins from Pairs polyphylla Smith var yunnanensis(Franch)Hara

[目的]建立滇重楼皂苷有效部位HPLC指纹图谱分析方法,研究不同产地滇重楼药材的质量。[方法]采用HPLC等度洗脱的方法进行色谱分离,使用"相似度评价软件"进行数据处理,对不同产地的滇重楼药材皂苷部位进行指纹图谱的研究。[结果]确立了滇重楼药材皂苷部位指纹图谱的高效液相分析条件,确定8个共有峰。不同产地的滇重楼药材皂苷部位指纹图谱有一定差异。[结论]采用HPLC法制定滇重楼药材皂苷部位的指纹图谱,其皂苷类成分得到较好的分离,方法重现性好,可用于滇重楼的质量评价。

[ Objective] The research aimed to establish a method of HPLC-fingerprint spectrum （HPLC-FPS） for the active part in Pairs polyphylla Smith var yunnanensis（Franch） Hara in order to control it＇s quality from different palaces.[Method] The isocratic elution mode was applied in chromatographic separation, and data were analysed by ＂Similarity Evaluation software＂ to compare it＇ s HPLC-FPS from different places. [Resuh]The conditions for HPLC analysis of Pairs polyphylla Smith var yunnanensis（Franch） Hara were established and 8 common peaks were separated. [ Conclusion] All components in the spectrum were separated well and the HPLC fingerprint method is repeatable. The method can be used in quality assessment of Pairs polyphylla Smith var yunnanensis（Franch） Hara