Survivin及其剪接变异体在宫颈病变组织中的表达研究

Survivin and its expression of splice variants in tissues of cervical lesion

目的:探讨survivin及其剪接变异体在宫颈上皮病变组织中的表达及意义。方法:采用RT-PCR方法检测10例宫颈炎、10例宫颈上皮内瘤变Ⅰ级(Cervical intraepithelialneoplasiaⅠ,CINⅠ)、20例CINⅡ～Ⅲ及20例宫颈鳞状上皮细胞癌(squamouscellcarcinoma,SCC)组织中survivin、survivin-2B、survivin-ΔEx3、survivin-2αmRNA表达。结果:survivin及其剪接变异体在SCC组织中表达普遍升高,其表达阳性率随宫颈上皮病变的进展逐渐升高。Spearman等级相关分析显示survivin、survivin-2B和survivin-ΔEx3的表达与宫颈上皮病变的级别呈高度正相关(rs=0.864、0.899、0.827,P<0.01),survivin-2α的表达与宫颈上皮病变的级别呈中度正相关(rs=0.612,P<0.01);survivin-2B、survivin-ΔEx3和survivin-2α的表达与survivin的表达呈高度正相关(rs=0.922、0.991、0.999,P<0.01)。结论:survivin剪接变异体伴随着survivin的表达在宫颈上皮病变中表达逐渐升高,可能与survivin一起共同参与了宫颈癌的发生、发展,有望成为宫颈癌基因治疗的新靶点。

Objective：To investigate the significance of survivin and its splice variants expression in distinct epithelial tissues from cervical lesion. Methods ： Technique of reverse transcriptase polymerase chain reaction （RT-PCR） was used to detect the expression of survivin, survivin- 2B, survivin-△Ex3, survivin-2α mRNA in 10 cases with cervicitis, another 10 of cervical intraepitbelial neoplasia Ⅰ （ CIN Ⅰ ）, 20 CIN Ⅱ - Ⅲ and 20 squamous cell carcinoma（SCC） tissues. Results ：The positive expression of survivin,survivin-2B,survivin-△Ex3 and survivin-2α mRNA gradually increased with further development of cervical iesions from cervicitis to CIN and ultimate SCC. The findings showed statistical difference when the elements were compared in pairs by Fisher exact probability test（ P2tail 〈 0.05 ）. Pearson correlation analysis suggested that the positive expression of the four factors were directly linked to the degree of cervical intraepithelial lesion （rs = 0. 864,0. 899 and 0. 827, P 〈 0. 01, respectively）. Also, the expression of splice variants was positively correlated with surviving of wild type（ r = 0. 922,0. 991 and 0. 999, P 〈 0.01, respectively）. Conclusion：The expression of splice variants increases with the progress of cervical lesion, suggesting that survivin may be a role in the development and progress of cervical cancer and is expected to be the new targets of gene therapy for the disease.