白癜风患者外周血CD4^＋CD25^＋调节性T细胞的检测

Detection of peripheral CD4^＋CD25^＋ regulatory T lymphocytes in patients with vitiligo

目的检测不同病期白癜风患者外周血CD4^＋CD25^＋调节性T细胞水平，探讨其与白癜风发病的关系。方法白癜风患者34例，进展期19例，稳定期15例。通过流式细胞仪对不同病期白癜风患者外周血CD4^＋,CD4^＋CD25^＋T细胞水平进行检测，并与20例正常人比较。结果进展期患者外周血中CD4^＋CD25^＋调节性T细胞占外周血淋巴细胞的表达率低于正常对照组（P〈0．05）；稳定期患者与正常对照组比，差异无统计学意义（P〉0．05）；进展期患者低于稳定期患者，差异有统计学意义（P〈0．05）。进展期患者CD4^＋CD25^＋调节性T细胞占外周血淋巴细胞表达率与皮损面积呈负相关（P〈0．05），稳定期则无相关性（P〉0．05）。进展期与稳定期患者CD4^＋CD25^＋调节性T细胞占外周血淋巴细胞水平与病程均无明显相关性（P〉0．05）。结论白癜风患者外周血中存在异常比例的CD4^＋CD25^＋调节性T细胞，可能与白癜风的发病有关。

Objective To determine the level of peripheral CD4^＋CD25^＋ regulatory T lymphocytes in patients with vitiligo at different stages and to study its relationship with the development of vitiligo. Methods Blood samples were collected from 34 outpatients with vitiligo, including 19 cases of progressive vitiligo and 15 cases of stable vitiligo, as well as from 20 normal human controls. Flow cytometry was used to detect the levels of peripheral CD4^＋ and CD4^＋CD25^＋T lyrnphocytes in these samples. Results Compared with the controls, the percentage of CD4^＋CD25^＋ regulatory T lymphocytes in peripheral lymphocytes was significantly lower in patients with progressive vitiligo than those in patients with stable vitiligo and normal human controls [（2.43 ± 0.30）% vs （3.49 ±0.39）% and （3.34 ±0.24）%, both P 〈 0.05], but no significant difference was found between patients with stable vitiligo and normal human controls （P 〉 0.05 ）. A significantly negative correlation was observed between the percentage of CD4^＋CD25^＋ regulatory T lymphocytes and lesion area in patients with progressive vitiligo （r = -0.48, P 〈 0.05 ）, but not in patients with stable vitiligo （P 〉 0.05）. There was no significant correlation between the course of disease and the percentage of peripheral CD4^＋CD25^＋ regulatory T lymphocytes in patients with progressive vitiligo or stable vitiligo （both P 〉 0.05 ）. Conclusion There is an abnormal proportion of peripheral CD4^＋CD25^＋ regulatory T lymphocytes in patients with vitiligo, which may be related to the development of vitiligo.