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针药合用刺激嗅觉系统对血管性痴呆大鼠学习记忆功能及大脑边缘叶自由基系统的影响 被引量:3

Effect of Combination of Acupuncture and Medicine on Learning-memory Ability and Free Radical System of Cerebral Marginal Lobe in Vascular Dementia Rats
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摘要 【目的】探讨针药合用刺激嗅觉系统对血管性痴呆(VD)大鼠学习记忆功能及大脑边缘叶自由基系统的影响。【方法】选用成年SD雄性大鼠48只,随机分为正常对照组、VD模型组、嗅三针组(针刺两侧迎香及印堂穴)、丁香酚刺激组、针药合用1组(先复制VD模型,然后损毁双侧嗅球,再行嗅三针和丁香酚刺激)及针药合用2组(仅复制VD模型,再行嗅三针和丁香酚刺激),每组8只。测试各组大鼠水迷宫学习记忆能力并测定大脑边缘叶丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性。【结果】学习记忆能力检测结果显示,大鼠6 d平均逃避潜伏期和平均游泳路程比较,VD模型组显著长于正常对照组(P<0.01);嗅三针组、丁香酚刺激组和针药合用2组显著短于VD模型组(P<0.01);针药合用2组短于嗅三针组、丁香酚刺激组(P<0.05)。嗅三针组与丁香酚刺激组比较、VD模型组与针药合用1组比较,差异均无显著性意义(P>0.05)。大脑边缘叶自由基系统检测结果显示,与正常对照组比较,VD模型组MDA含量显著增高(P<0.01),而GSH-Px和SOD活性显著降低(P<0.01);与VD模型组比较,嗅三针组、丁香酚刺激组和针药合用2组MDA含量显著降低(P<0.01),而GSH-Px和SOD活性显著增高(P<0.01);针药合用2组与嗅三针组、丁香酚刺激组比较,MDA含量降低(P<0.05),而GSH-Px和SOD活性增高(P<0.05);嗅三针组与丁香酚刺激组比较、VD模型组与针药合用1组比较,MDA含量及GSH-Px、SOD活性差异均无显著性意义(P>0.05)。【结论】嗅三针和丁香酚均能够显著增强VD大鼠学习记忆功能,降低大脑边缘叶MDA的含量,提高大脑边缘叶GSH-Px和SOD的活性,针药合用具有一定协同作用,其治疗效应的发挥依赖于嗅觉传导通路的完整性。 Objective To investigate the effect of stimulating olfactory system with acupuncture and medicine on learning-memory abilities and free radical system of cerebral marginal lobe in vascular dementia (VD) rats. Methods Forty-eight SD male rats were equally randomized into 6 groups: normal group, model group, acupuncture group (acupuncture on acupoints of bilateral Yingxiang and Yintang), eugenol group, combination group 1 (stimulation with acupuncture and eugenol on the VD rats with removal of bilateral olfactory bulbs) and combination group 2 ( stimulation with acupuncture and eugenol on the VD rats). VD models were established by 4-vessel occlusion. Morris maze tests were conducted for the evaluation of rats learning and memory abilities. The malondaldehyde (MDA) content, and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in cerebral marginal lobe were detected. Results The results of Morris maze tests showed that average escape latency and travel distance were prolonged in the model group compared with the normal group ( P 〈 0.01 ), and were shortened in the acupuncture group, eugenol group and combination group 2 compared with the model group (P 〈 0. 01 ). The average escape latency and travel distance were shorter in the combination group 2 than those in the acupuncture group and eugenol group ( P 〈 0.05 ), and the difference was insignificant between the acupuncture group and eugenol group, and insignificant between the model group and the combination group 1 (P 〉 0. 05 ). In comparison with the normal group, MDA content was increased, and GSH-Px and SOD activities were decreased in the model group ( P 〈 0. 01 ). MDA content was decreased, and GSH-Px and SOD activities were increased in the acupuncture group, eugenol group and combination group 2 (P 〈 0.01 compared with the model group) ; the changes of above parameters were obvious in the combination group 2 ( P 〈 0. 05 ). The differences of the above three parameters were insignificant between the acupuncture group and eugenol group, and insignificant between the model group and the combination group 1 ( P 〉 0. 05 ). Conclusion Stimulation with eugenol and acupuncture on acupoints of bilateral Yingxiang and Yintang can improve the learning and memory abilities, reduce MDA content, and increase GSH-Px and SOD activities in cerebral marginal lobe of vascular dementia rats, indicating that the combination of acupuncture and medicine has synergistic action, and the achievement of therapeutic effect depends on the integrity of olfactory pathway.
出处 《广州中医药大学学报》 CAS 2009年第4期351-354,共4页 Journal of Guangzhou University of Traditional Chinese Medicine
基金 陕西省科学技术研究发展计划项目[编号:2007K13-03(17)]
关键词 血管性痴呆/针灸疗法 血管性痴呆/中药疗法 自由基 疾病模型 动物 大鼠 迎香 印堂 VASCULAR DEMENTIA/acup-mox therapy VASCULAR DEMENTIA/TCD therapy FREE RADICALS DISEASE MODELS, ANIMAL RATS POINT LI20 (YINGXIANG) POINT EX-HN3 (YINTANG)
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