胶体金免疫层析法快速检测组织样品中喹诺酮类药物残留

Rapid detection of quinolones residue in tissue through colloidal gold immunochromatographic assay

目的：建立用于快速检测组织样品中喹诺酮类药物残留的胶体金免疫层析法。方法：采用免疫竞争法,将抗喹诺酮类单克隆抗体-胶体金复合物包被在胶体金结合垫上,并将人工合成的喹诺酮类抗原包被在硝酸纤维素薄膜表面作为检测线（T线）,其与待测样品中相应喹诺酮类药物竞争结合胶体金标记的抗喹诺酮类单克隆抗体,并能以颜色直观显示检测的定性结果。结果：检测肉、鱼、虾等组织试样时,灵敏度最低值可达到10 ng/ml,只需3~5 min,与类似物无交叉反应。结论：该试剂具有较高的灵敏度及特异性,操作便捷,稳定可靠,可作为喹诺酮类残留现场监控的有效筛检手段。

Objective：A rapid, simple colloidal gold immunochromatographic assay for detecting quinolones （QNs）residues has been established. Methods： Nanocolloidal gold particles were prepared and labeled to an anti - quinolones monoclonal anti- body. QNs was conjugated to bovine serum albumin （BSA） and dispersed on a nitrocellulose （NC） membrane to be the test line （T）. The more analyte present in the sample, the more effectively it would compete with the QNs - BSA for binding to the limited amount of gold - labeled anti - quinolones monoclonal antibody. The presence or absence of a colored band on the test line indi- cates a negative or positive result. Results：When measuring the water sample spiked with quinolones, the minimum detection concentration can reach 10 ng/ml. The major advantage of the one - step strip test is that the test can be completed within 5 minutes. No cross -reaction occurred between quinolones and other veterinary drugs. Conclusion：The method could be widely used to detect quinolones residue on spot because of its high sensitivity, specificity and good stability.