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腹腔感染大鼠肠黏膜屏障功能障碍与热休克蛋白-70表达的研究

Research of the relationship between intestinal mucosal barrier dysfunction and the expression of heat shock protein 70 of abdominal infected rats
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摘要 目的观察人参皂甙Rb1针剂(Ginsenoside Rb1,GRb1)对腹腔感染大鼠肠黏膜细胞热休克蛋白-70(HSP-70)的影响,探讨GRb1对肠黏膜屏障的保护作用机制。方法30只SD雄性大鼠随机分为正常组、对照组及实验组,各10只。对照组及实验组建立腹腔感染模型。实验组于造模前7d予GRb1针剂(20mg/kg),其他组予等量生理盐水,每天腹腔注射1次。各组造模后42h,管饲已标记的大肠杆菌。48h处死后,取小肠作病理学分析及免疫组织化学检测小肠黏膜HSP-70的表达;取胰腺、肾脏、脾脏、肝脏组织,检测大鼠移位标记菌变化。结果与对照组相比,GRb1能明显提高腹腔感染大鼠小肠黏膜的HSP-70表达,并减轻小肠黏膜组织破坏程度,减少胰腺、肾脏、脾脏、肝脏等周围器官的细菌移位。结论GRb1能提高肠黏膜细胞HSP-70的表达,减轻其肠黏膜损伤,减少细菌移位,具有一定的肠黏膜屏障保护作用。 Objective Exploration of enhancing intestinal mucosa cells HSP70 expression can protect intestinal mucosal barrier. Methods 30 SD male rats were randomly divided into the normal group, the control group and the treatment group. The latter 2 groups were supposed to be establishment of abdominal testis model. In the seven days before the start of the day by intraperitoneal injection once a treatment group to ginsenoside Rbl injection, the other groups were given equal saline. After 42 hours of the establishment of abdominal testis model, marked Bacterium coli were fed. After 48 hours, the rats were killed. Keep the small intestine for pathologic analysis and immunohistochemistry mucosa of heat shock protein 70 expression. Keep the pancreas, kidney, spleen, liver tissue to detect bacterial translocation. Results Compared to the control group, Ginsenoside Rbl can significantly increase intra-abdominal infection of the small intestinal expression of heat shock protein 70, and reduce the organizational structure of the small intestinal damage and bacterial translocation of pancreas, kidney, spleen, liver. Conclusions Increasing the expression of HSP70, of the intestinal cells' can reduce the organizational structure of the small intestinal damage and bacterial translocation. It can protect the intestinal mucosal barrier.
出处 《现代实用医学》 2009年第6期555-557,560,F0002,共5页 Modern Practical Medicine
基金 浙江省医药卫生科学研究基金 编号:2002A070
关键词 热休克蛋白 腹腔 感染 肠黏膜 abdominal infection, intestinal mucosal barrier, heat shock protein 70, Ginsenosides Rb1
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