摘要
目的建立绝经后骨质疏松合并动脉粥样硬化大鼠实验模型。方法在去势的基础上3次大剂量肌肉注射维生素D与高脂灌胃相结合处理大鼠,90天后观察血脂,胸主动脉的形态学变化,取左侧胫骨行骨组织形态计量学测量,右侧股骨进行骨密度的测定,测定后行三点弯曲试验,左侧股骨行骨矿物元素测定。结果在去卵巢的基础上3次大剂量肌肉注射维生素D与高脂灌胃后,大鼠血清TC、LDLC均明显升高(P〈0.01);HDLC明显下降(P〈0.01);主动脉形成较成熟的动脉粥样斑块;大鼠骨骨小梁面积百分率、骨小梁厚度、骨小梁数量均显著下降(P〈0.01)、骨小梁分离度明显升高(P〈0.05),骨密度、最大载荷、弹性载荷、弹性挠度、断裂载荷、骨钙、骨镁、骨磷、骨羟脯氨酸含量均显著下降(P〈0.05)。结论采用在去卵巢的基础上3次大剂量肌肉注射维生素D与高脂灌胃相结合的方法可以成功建立骨质疏松并发AS大鼠模型。
Objective To develop a model of osteoporosis with atherosclerosis plaque in rats. Methods The rats were performed ovariectomized (OVX) operation and fed with high-cholesterol (HC) diet plus vitamin D3 (VD) intramuscular injection for 90 days. At endpoint the serum lipid, morphology change of thoracic aorta were determined. The right femur were tested bone mineral density ( BMD), then conducted biomechanical property assay. The bone calcium content and bone hydroxyproline content of left femur were also determined. Results The serum total cholesterol ( TC), low-density lipoprotein cholesterol (LDLC) were significant increased and high density lipoprotein cholesterol ( HDLC ) decreased in OVX + HC + VD group when compared with those in sham and in OVX alone (P 〈0. 01 ) The atheromatous plaques were presented in the rats treated with OVX + HC + VD; compared with sham group, the percent trabecular area ( % Tb. Ar) , trabecular thickness (Tb. Th), trabecular number ( Tb. N) were decreased ( P 〈 0. 01 ), and trabecular separation (Tb. Sp) was increased (P 〈 0. 05 ), and the BMD, max-load, elasticity-load, break-load, breakstain of right femur were decreased (P 〈 0. 05). The contents of Ca, P and hydroxyproline of left femur were decreased both in OVX and in OVX + HC + VD group (P 〈 0.05). Conclusions Rat can be used as an ideal model for research osteoporosis with atherosclerosis. The osteoporosis with atheromatous plaque may created by OVX + HC + VD.
出处
《中华骨质疏松和骨矿盐疾病杂志》
2009年第2期127-133,共7页
Chinese Journal Of Osteoporosis And Bone Mineral Research
基金
国家自然基金资助项目(No:30672470)