期刊文献+

正丁基-β-D-吡喃果糖苷体外对Bel-7402细胞凋亡的诱导作用 被引量:6

Induction effect of n-butyl-β-D-fructosidepyranose on apoptosis bel-7402 cells in vitro
原文传递
导出
摘要 目的:探讨正丁基-β-D-吡喃果糖苷诱导肝癌Bel-7402细胞凋亡的作用机制。方法:采用四甲基偶氮唑蓝(MTT)比色法,检测正丁基-β-D-吡喃果糖苷对Bel-7402细胞的增殖抑制效应;将6.25,12.5,25mg·mL-1的正丁基-β-D-吡喃果糖苷作用于Bel-7402细胞24h和48h,采用流式细胞术(FCM)测定细胞周期及凋亡率;应用凝胶成像分析仪检测DNALadder。结果:正丁基-β-D-吡喃果糖苷在体外对Bel-7402细胞有增殖抑制效应,在正丁基-β-D-吡喃果糖苷作用下,Bel-7402细胞出现显著的细胞凋亡征象;流式细胞术检测结果显示,不同浓度的正丁基-β-D-吡喃果糖苷与Bel-7402细胞作用24,48h可明显抑制Bel-7402细胞存活,25mg·mL-1的正丁基-β-D-吡喃果糖苷抑制率达到42.39%;凝胶成像分析仪检测到典型的DNA阶梯状条带。结论:正丁基-β-D-吡喃果糖苷有诱导Bel-7402细胞凋亡的作用,且随着浓度的升高和作用时间的延长,其诱导细胞凋亡作用增强。 OBJECTIVE To investigate induction effect of n butyl -β-D-fructosidepyranose on apoptosis in human hepatocellular carcinoma and its mechanisms in vitro. METHODS Bel-7402 cells were treated with n-butyl-β-D-fructosidepyranose (6. 25,12.5,25 mg·L^-1), The proliferation inhibition was examined by MTT assay. Cell apoptosis and cell cycle were analyzed by flow cytometry (FCM). DNA Ladder was detected with gel imaging analysator. RESULTS When treated for 24 h and 48 h, the apoptosis was obviously inhibited by the different concentration of n-butyl-β-D-fructosidepyranose, and the apoptosis rate of 25 mg·L^-1 n-butyl-β-D-fructosidepyranose was 42. 39%. DNA Ladder was obviously observed with the gel imaging analysator. CONCLUSION The n-butyl-β-D-fructosidepyranose could induce the Bel-7402 cell apoptosis, and the cell apoptosis was streghened with the increasing concentration of n butyl-β-D-fructosidepyranose and the time of action.
出处 《中国医院药学杂志》 CAS CSCD 北大核心 2009年第15期1253-1255,共3页 Chinese Journal of Hospital Pharmacy
基金 国家自然科学基金项目(编号:30870249 30772761)
关键词 正丁基-β-D-吡喃果糖苷 流式细胞仪 细胞凋亡 BEL-7402细胞 肝癌 琼脂糖凝胶 n-butyl-β-D-fructosidepyranose flow cytometry apoptosis Bel-7402 cells hepatoma agarose gel
  • 相关文献

参考文献7

二级参考文献97

共引文献37

同被引文献57

  • 1黄雪峰,罗俊,张勇,孔令义.石刁柏的木脂素类成分研究[J].中国药学杂志,2007,42(5):339-340. 被引量:3
  • 2张宇,吴莉莉,李金伟.3种十字花科芸苔属植物化学成分研究[J].黑龙江医药科学,2007,30(2):21-22. 被引量:7
  • 3蔡华芳.鲜竹沥镇咳祛痰作用的实验研究[J].中国实验方剂学杂志,2007,13(5):43-44. 被引量:26
  • 4Editorial Board of Zhong-Hua-Ben-Cao( China Herbal), State Administration of Traditional Chinese Medicine (国家中医药管理局<中华本草>编委会).China Bencao (中华本草). Shanghai : Shanghai Science & Technology Press, 1999. 460.
  • 5Guangzhou Forces General Logistics Department of Health (广州部队后勤部卫生部).Chinese Herbal Medicin Handbook(常用中草药手册).Beijing:People's Health Publishing House,1969:772-773.
  • 6Juangsu New Medical College(江苏新医学院).Dictionary of Chinese Herbal Medicine(中药大辞典,下),Shanghai:Shanghai Science&Technology Press,2006.3430.
  • 7Cai H,Al-Fayez M,Tunstall RG,et al.The rice bran constituent tricin potently inhibits cyclooxygenase enzymes and interferes with intestinal carcinogenesis in ApcMin mice.Mo Cancer Ther,2005,4:1287-1292.
  • 8Rocha AB,Lopes RM,Schwartsmann G.Natural products in anticancer therapy.Curr Opin Pharmacol,2001,1:364-369.
  • 9Sartippour MR,Liu CH,Shao ZM,et al.Livistona extract inhibits angiogenesis and cancer growth.Oncol Rep,2001,8:1355-1357.
  • 10Cheung Susan,Tai Joseph.In vitro studies of the dry fruit of Chinese fan palm Livistona Chinensis.Oncol Rep,2005,14:1331-1336.

引证文献6

二级引证文献52

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部