摘要
目的:探讨正丁基-β-D-吡喃果糖苷诱导肝癌Bel-7402细胞凋亡的作用机制。方法:采用四甲基偶氮唑蓝(MTT)比色法,检测正丁基-β-D-吡喃果糖苷对Bel-7402细胞的增殖抑制效应;将6.25,12.5,25mg·mL-1的正丁基-β-D-吡喃果糖苷作用于Bel-7402细胞24h和48h,采用流式细胞术(FCM)测定细胞周期及凋亡率;应用凝胶成像分析仪检测DNALadder。结果:正丁基-β-D-吡喃果糖苷在体外对Bel-7402细胞有增殖抑制效应,在正丁基-β-D-吡喃果糖苷作用下,Bel-7402细胞出现显著的细胞凋亡征象;流式细胞术检测结果显示,不同浓度的正丁基-β-D-吡喃果糖苷与Bel-7402细胞作用24,48h可明显抑制Bel-7402细胞存活,25mg·mL-1的正丁基-β-D-吡喃果糖苷抑制率达到42.39%;凝胶成像分析仪检测到典型的DNA阶梯状条带。结论:正丁基-β-D-吡喃果糖苷有诱导Bel-7402细胞凋亡的作用,且随着浓度的升高和作用时间的延长,其诱导细胞凋亡作用增强。
OBJECTIVE To investigate induction effect of n butyl -β-D-fructosidepyranose on apoptosis in human hepatocellular carcinoma and its mechanisms in vitro. METHODS Bel-7402 cells were treated with n-butyl-β-D-fructosidepyranose (6. 25,12.5,25 mg·L^-1), The proliferation inhibition was examined by MTT assay. Cell apoptosis and cell cycle were analyzed by flow cytometry (FCM). DNA Ladder was detected with gel imaging analysator. RESULTS When treated for 24 h and 48 h, the apoptosis was obviously inhibited by the different concentration of n-butyl-β-D-fructosidepyranose, and the apoptosis rate of 25 mg·L^-1 n-butyl-β-D-fructosidepyranose was 42. 39%. DNA Ladder was obviously observed with the gel imaging analysator. CONCLUSION The n-butyl-β-D-fructosidepyranose could induce the Bel-7402 cell apoptosis, and the cell apoptosis was streghened with the increasing concentration of n butyl-β-D-fructosidepyranose and the time of action.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2009年第15期1253-1255,共3页
Chinese Journal of Hospital Pharmacy
基金
国家自然科学基金项目(编号:30870249
30772761)