曲尼司特对大鼠主动脉平滑肌细胞增殖的影响

Effect of tranilast on proliferation in rat aortic smooth muscle cell

目的：为探讨曲尼司特预防再狭窄的可能性，研究了曲尼司特对培养的大鼠主动脉平滑肌细胞（ＡＳＭＣ）增殖的影响。方法：以培养的大鼠ＡＳＭＣ为模型，应用＾３Ｈ－ＴｄＲ掺入试验观察ＡＳＭＣ增殖情况。结果：曲尼司特呈剂量信赖性无抑制基础、血小板源生长因子（ＰＤＧＦ）和血管紧张素Ⅱ（ＡｎｇⅡ）诱导的ＡＳＭ ＤＮＡ合成，与相应对照组比较Ｐ〈０．０５或Ｐ〈０．０１。结论：曲尼司特能明显抑制ＡＳＭＣ增殖。

Objective:To investigate the possibility of tranilast on the prevention of restenosis,we examined the ef- fect of tranilast on the proliferation of rat aortic smooth muscle cell(ASMC) cultured in vitro.Methods:Rat ASMC mod- el cultured in vitro was established.H-thymidine(~3H-TdR)incorporation test was employed to detect proliferation of ASMC.Results:Tranilast inhibited basal rat ASMC DNA synthesis,also markedly blunted both platelet-derived growth factor(PDGF) and Angiotensin Ⅱ(Ang Ⅱ)which induced increase of ASMC DNA synthesis in a dose-dependent manner (P<0.05 or 0.01 vs control).Conclusion:Tranilast can markedly inhibit ASMC proliferation,and may be beneficial in the prevention of restenosis.