自发性高血压大鼠降钙素基因相关肽水平观察

Calcitonin Gene-Related Peptide Levels in Spontaneously Hypertensive Rat

目的探讨降钙素基因相关肽（ＣＧＲＰ）在高血压的发生、发展中的病理生理意义。方法采用放射免疫分析法测定常压大鼠（ＷＫＹ）和自发性高血压大鼠（ＳＨＲ）血浆、脑脊液、脑及主动脉血管中ＣＧＲＰ含量。结果ＳＨＲ血浆ＣＧＲＰ水平（３５．５±７．２５ｎｇ／Ｌ）较ＷＫＹ大鼠（４９．６±１０．３ｎｇ／Ｌ）显著降低（Ｐ＜０．０１）；脑脊液中含量分别为４８．７±９．４与５０．３±１０．５ｎｇ／Ｌ，两者差异无显著性（Ｐ＞０．０５）；ＳＨＲ脑、主动脉血管组织ＣＧＲＰ含量分别为１０３．０±１７．４，６９．３±１９．３ｎｇ／ｇ，较ＷＫＹ大鼠（６２．３±１５．８，３１．３±２４．４ｎｇ／ｇ）显著升高（Ｐ＜０．００１；Ｐ＜０．００５）。结论ＳＨＲ体内ＣＧＲＰ水平的变化可能与高血压的发生。

Aim\ To discuss the pathological significance of calcitonin gene-related peptide(CGRP) in the occurence and development of hypertension.\ Metnods\ The changes of CGRP levels in the plasma, cerebrospinal fluid, the brain and the aorta of spontaneously hypertensive rats(SHR) and Wistar Kyoto rats(WKY) were measured by radioimmunoassay.\ Results\ The plasma CGRP levels in SHR(355±725 ng/L) were much lower(P<001) than those in WKY(496±103 ng/L); the CGRP levels in cerebrospinal fluid in SHR(487±94 ng/L) were somewhat lower than in WKY(503±105 ng/L), but with no statistical difference(P>005); while the CGRP levels in SHRs' brain(1030±174 ng/g) and aorta(693±193 ng/g) were higher(P<0001, P<001) than those in WKY(623±158 ng/g, 313±244 ng/g).\ Conclusions\ The changes of the CGRP levels in SHR might be closed related to the pathogenesis of spontaneously hypertension.