离子交换法分离提取谷氨酸转化液中的γ-氨基丁酸

Isolation and Purification of γ-aminobutyric Acid from Reaction Solution of Glutamic Acid by Ion-exchange Resins

采用固定化谷氨酸脱羧酶转化谷氨酸生成γ-氨基丁酸,从4种阳离子交换树脂中筛选出交换容量最高的D061型,用于分离谷氨酸转化液中的γ-氨基丁酸。用静态和动态的方法,考察了不同操作条件对固定床离子交换效果的影响。结果表明,D061型树脂最佳吸附条件为pH=4.0,温度40℃,平衡吸附时间17min,吸附量为0.83mol/L。谷氨酸转化液上样的最佳流速为1BV/h。洗脱剂氨水浓度为1.2mol/L,最优流速为1BV/h。经旋转蒸发仪浓缩、醇沉结晶后,可得γ-氨基丁酸样品,其回收率达到80%。样品由X射线衍射图谱进行了验证。

The immobilized glutamate decarboxylase can transform glutamic acid into γ-aminobutyric acid. Four types of acid cation resins were selected to separate γ-aminobutyrie acid from the transformation reaction solution of glutamic acid, in which D061 has the maximal γ-aminobutyric acid exchange capacity. Through static and dynamic experiments, different operating conditions in the fixed bed to separate γ-aminobutyric acid were investigated. The results showed that the optimum parameters were as follows： temperature at 40 ℃, adsorption balance time 17 min and pH 4 in the static experiment. The dynamic experimental results showed that the optimal adsorptive flow rate was 1 BV/ h in a column. The elution conditions were optimized that 1.2 mol/L of aqueous ammonia brought an efficient elution at a flow rate of 1.0 BV/h. The white needle crystal of γ-aminobutyric acid was obtained after the effluent of γ-aminobutyrie acid was concentrated, crystallized and dried. The total recovery rate was 80%. The sample was testified by X-ray diffraction patterns.