摘要
目的以来源广泛、原植物药用成分量可测定的刺五加叶柄为外植体,研究刺五加体细胞胚胎发生情况,为实现药用成分高产刺五加的快速繁殖奠定基础。方法以3年生植株萌发15 d以内的刺五加复叶叶柄为外植体,考察2,4-D和BA对刺五加体细胞胚胎发生的影响。结果28 d培养后,2,4-D 1.5 mg/L+BA 1.0 mg/L处理的叶柄外植体中71.4%直接产生或经由愈伤组织间接产生了8.5个体胚。两种产生方式均可在诱导培养基中进行,但间接发生比例较少。转入相同或降低2,4-D质量浓度的培养基后,体胚渐次发育成熟。同时新的体胚也在逐渐产生,经由间接途径产生的体胚所占比例也随之上升。结论刺五加可以通过萌发15 d以内的复叶叶柄外植体实现体细胞胚胎发生,体胚诱导率取决于2,4-D和BA的质量浓度。
Objective The petiole of Eleutherococcus senticosus was used as somatic embryo, which is widely original plants with the pharmacological active components whose contents could be determined, the somatic embryo in E. senticosus was studied, the aim of this study is to provide the proof of E. senticosus species which have the higher yield of pharmacological active components. Methods Using the petiole of E. senticosus of three years old plants germinated somatic embryos within 15 d to observe the somatic embryogenesis of E. senticosus with the 2,4-D+BA medium. Results After cultured for 28 d with 2,4-D 1.5 mg/L+BA 1.0 rag/L, 71.4% of the petiole somatic embryos were directly produced or 8.5 embryos in total were produced via callus. Both of the two methods could be used in the elicitation medium, but the percentage of indirect production was smaller. After transforming into the same or the lower concentration of 2,4-D medium, the somatic embryos gradually matured. At the same time, those of the new somatic embryos were also produced, the percentage of the somatic embryos which were produced by indirect way was increased with it. Conclusion Using the petiole of E. senticosus germination within 15 d could make somatic embryogenesis. It confirms that the somatic embryogenesis and the bodybmeryos inductivity depend on the 2, 4-D and BA concentration.
出处
《中草药》
CAS
CSCD
北大核心
2009年第8期1302-1305,共4页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30701086)
关键词
刺五加
叶柄
体细胞胚胎
2
4-D
BA
Eleutherococcus senticosus Harms
petiole
somatic embryos
2,4-D
BA