丝氨酸蛋白酶对心血管涤纶生物材料上细菌生物膜形成影响的研究

Effect of SspA on the Formation of Bacterial Biofilm Covering the Surfaces of Cardiovascular Biomaterial Dacron

研究丝氨酸蛋白酶(SspA)对心血管涤纶生物材料上细菌生物膜(Bacterial biofilm,BF)形成的影响。自金黄色葡萄球菌(Staphylococcus aureus,SA)膜表面提取、纯化SspA,用于影响SA在涤纶生物材料(Dacron)上的细菌黏附和BF的形成。利用激光共聚焦扫描显微镜(Confocal laser microscopy,CLSM)测定不同浓度梯度下SspA对SA在心血管涤纶生物材料表面BF的形成。发现SspA能有效抑制SA在心血管生物材料涤纶片上BF的形成,其抑制作用的强度与SspA剂量呈正相关性。实验表明SspA的作用可为临床治疗以生物材料为中心的感染(Bio-material centered infection,BCI)提供帮助。

This study sought to assess the effect of SspA on the formation of Staphylococcus aureus biofilm extending over the surfaces of Cardiovascular Biomaterial Dacron. SspA was extracted from the surface of staphylococcus aureus biofilm, purified, and then used to influence the adhesion of Staphylococcus aureus and the formation of Staphylococcus aureus biofilm on Dacron biomaterial surfaces. The formation of the Staphylococcus aureus biofilm on cardiovascular biomaterial Dacron surfaces under gradient SspA concentrations was evaluated by confocal laser microscopy. The result revealed that SspA inhibited the formation of Staphylococcus aureus biofilms on cardiovascular biomaterials surfaces effectively, and it was dose dependent. This study indicates that SspA is effective for preventing biomaterial centered infection and this method is conducive to clinical applications.