蜕皮甾酮对胰岛素抵抗HepG2细胞胰岛素受体底物表达的影响

Effect of Ecdysterone on Protein Expression of IRS-1/-2 in Insulin-resistant HepG2 Cells

目的:探讨蜕皮甾酮对胰岛素抵抗(IR)HepG2细胞胰岛素受体底物(IRS)蛋白表达的影响。方法:胰岛素抵抗HepG2细胞模型建立后,培养液中加入蜕皮甾酮共同孵育,观察蜕皮甾酮及吡格列酮对模型细胞葡萄糖掺入率的影响;应用免疫细胞化学染色法等方法观察蜕皮甾酮对胰岛素抵抗HepG2细胞IRS-1I、RS-2表达的影响。结果:与模型细胞组比较,1×10-5mol/L蜕皮甾酮可使IR HepG2细胞IRS-1I、RS-2蛋白的表达显著增加。结论:蜕皮甾酮的胰岛素增敏作用可能与胰岛素信号转导分子IRS-1I、RS-2蛋白的表达增强有关。

Objective： To investigate the effect of ecdysterone on protein expression of IRS-1/-2 in insulin-resistant HepG2 cells. Methods： After establishment of the insulin resistant HepG2 cell model induced by high concentration of insulin, and the HepG2 cells were incubated with 1× 10^-5 mol/L ecdysterone for 24 h. Immunocytochemistry assay was used to assess the expression of IRS-1 and IRS-2. Results： The incubation of insulin resistance cells with ecdysterone 1× 10^-5 mol/L could significantly increase the glucose uptake in IR model cells. The protein contents of IRS-1 and IRS-2 by immunocytochemistry assay was increased compared with IR model cells. Conclusion： Ecdysterone could improve the insulin sensitivity in IR HepG2 cells probably by upregulating the expression of insulin signaling proteins such as IRS-1 and IRS 2.