摘要
以马铃薯品种黄麻子和中薯3号脱毒微型薯为外植体,利用根癌农杆菌介导法,将来源于诸葛菜的抗逆相关转录因子ODREB2B基因导入马铃薯,并对遗传转化的影响因素进行优化。获得抗性植株黄麻子33株、中薯3号27株,经PCR-Southern杂交分析,两品种分别有16株和14株呈阳性,证明ODREB2B基因已整合到马铃薯基因组中。
ODREB2B gene was originated from Orychophragmus vidaceus which exist in adverse resistance transcription factor. In this study, ODREB2B gene was transferred into the potato euhivars ＇ Huangmazi＇ and ＇ Zhongshu No. 3 ＇ by Agrobacterium-mediated transformation, and influence factors of genetic transformation were optimized. Microtuber discs were used as explants. 33 resistant plants of ＇Huangmazi＇ and 27 resistant plants of ＇ Zhongshu No. 3＇ were obtained. Resistant plants were blot analysis by PCR-Southern, of which 16 and 14 plants had a positive amplification. PCR-Southern blot showed that single copies of the ODREB2B gene were inserted into the genome of the transgenie plants.
出处
《中国蔬菜》
北大核心
2009年第16期20-25,共6页
China Vegetables
基金
黑龙江省"十一五"科技攻关重大项目(GA06B103-2)
