野生型P53和C-Ha-ras基因在顺铂诱导膀胱癌细胞凋亡中的表达及意义

The expression of wildtype P53 and CHaras in bladder cancer cells with apoptosis induced by cisplatin (CDDP)

应用荧光原位杂交技术观察顺铂诱导膀胱癌细胞株ＢＩＵ８７体外细胞凋亡过程中野生型Ｐ５３和ＣＨａｒａｓ基因的表达，探讨抗癌药物诱导膀胱癌细胞凋亡过程中抗癌基因和癌基因的作用。结果显示：顺铂能够明显地诱导膀胱癌细胞凋亡，具有一定范围内的作用时间和药物浓度的依赖性。随凋亡细胞数上升，野生型Ｐ５３表达增强，而ＣＨａｒａｓ基因表达下降，提示膀胱癌细胞凋亡可能是顺铂的杀瘤效应途径之一。Ｐ５３和ＣＨａｒａｓ基因均参与了细胞凋亡的调控过程，野生型Ｐ５３基因可能是通过下调ＣＨａｒａｓ基因的表达而发挥其抑制细胞增殖活性，促进凋亡的发生。

The expression of P53 and CHaras of BIU87 bladder cancer cell lines with apoptosis induced by CDDP was detected by means of fluorescence immuno in situ hybridization (FISH).An extensive apoptosis in BIU87 cells treated by CDDP has occured, which was dependent on an apropriate concentration of the drug or treatment time. The positive expression rate of P53 has been significantly upregulated and CHaras was downregulated as apoptotic index increased. These results suggested enhanced expression of P53 or decreased expression of CHaras might be involved in apoptosis or inhibition of the proliferation of bladder cancer cells induced by CDDP.