Mithramycin A对大肠癌细胞株SW620表达OPN及Sp1的影响

Effects of Mithramycin A on the expression of osteopontin and Sp1 in the colorectal carcinoma cell SW620

目的了解Sp1特异性抑制剂Mithramycin A对大肠癌细胞株SW620表达骨桥蛋白OPN的影响。方法以体外建系的大肠癌细胞株SW620为研究对象,在含有Mithramycin A的培养皿中孵育,细胞免疫荧光染色分析OPN在干扰前后的变化。结果Mithramycin A作用于细胞株SW620后,OPN及Sp1表达下调。结论大肠癌细胞株SW620表达OPN与转录因子Sp1相关。

Objective To explore the expression of osteopontin （OPN） in the colorectal carcinoma cell line SW620 before and after treatment with Mithramycin A. Methods Taken established human colorectal cancer cell line SW620 in vitro as research object, cellular immunofluorescence technique were used to detect the OPN expression at protein expression level. Results The level of OPN expression in cell line SW620 was down-regulated after treatment with Mithramycin A （P〈0.05）. Conclusion The expression of Sp1, OPN is significantly correlated in cell line SW620.