细胞因子对甲状腺相关性免疫眼眶病发病机制的研究

A study of cytokines in pathogenesis of thyroid-related immune orbitopathy

目的探讨细胞因子在甲状腺相关性免疫眼眶病（ｔｈｙｒｏｉｄｒｅｌａｔｅｄｉｍｍｕｎｅｏｒｂｉｔｏｐａｔｈｙ，ＴＲＩＯ）发病机制中的作用。方法体外培养眶成纤维细胞，应用免疫组化，观察细胞因子对眶成纤维细胞人类白细胞表面抗原Ⅱ（ｈｕｍａｎｌｅｕｋｏｃｙｔｅａｎｔｉｇｅｎⅡ，ＨＬＡⅡ）异常表达的诱导作用。结果干扰素γ（ｉｎｔｅｒｆｅｒｏｎγ，ＩＦＮγ）、白细胞介素１（ｉｎｔｅｒｌｅｕｋｉｎ１，ＩＬ１）及肿瘤坏死因子（ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ，ＴＮＦ）对眶成纤维细胞ＨＬＡⅡ抗原异常表达有诱导作用，ＩＦＮγ的诱导作用最强且与时间和浓度呈正相关。结论细胞因子（ＩＦＮγ，ＩＬ１，ＴＮＦ等）可诱导眶成纤维细胞ＨＬＡⅡ抗原异常表达，在启动免疫反应及甲状腺相关性免疫眼眶病发病机制中起重要作用。

Objective To explore the pathogenesis of thyroidrelated immune orbitopathy (TRIO). Methods Human orbital fibroblasts were cultured in vitro and immunohistochemical techniques were used to study cytokines that induce effect on HLAII antigen expression of orbital fibroblasts. Results It is shown that interferonγ (IFNγ), interleukin1 (IL1) and tumor necrosis factor (TNF) could induce the expression of orbital fibroblast HLAII antigens. The induced effect of IFNγ is of good linear correlations with time and concentration. Conclusion HLAII antigen expression can be induced by IFNγ, IL1 and TNF. In the course of immune reaction and pathogenesis of TRIO, cytokines play important roles. 