上皮性卵巢癌患者血清中骨桥蛋白及CA125检测的诊断、监测价值研究

Detection of osteopontin and CA125 in Patients with ovarian cancer and Its Clinical significance

目的探讨骨桥蛋白OPN作为卵巢上皮性卵巢癌标志物及联合检测CA125对卵巢上皮癌的临床诊断、病情监测的价值。方法采集卵巢上皮癌患者52例、卵巢上皮类良性肿瘤患者50例及健康妇女50例的血清样品,分别用酶联免疫吸附法及微粒子化学发光法测定OPN和CA125含量。结果卵巢上皮癌患者血清OPN和CA125的含量明显高于卵巢上皮类良性肿瘤组和健康妇女组。OPN检测敏感度、特异度分别为84.6%、94.0%,CA125检测敏感度、特异度分别为75.0%、90.0%,联合检测敏感度、特异度为92.1%、94.0%。卵巢上皮癌患者术前血清OPN和CA125明显高于术后,Ⅲ期卵巢癌患者的OPN和CA125明显高于Ⅰ、Ⅱ期。结论OPN是一种较为理想的卵巢上皮癌肿瘤标志物,其血清水平可作为卵巢上皮癌诊断和病情监测的临床指标,联合CA125检测对提高上皮性卵巢癌的诊断有一定的价值。

Objective To investigate the clinical value of osteopontin OPN as a tumor marker and combined detection of the serum levels of OPN and CA125 in diagnosing and monitoring of epithelium ovarian cancer. Methods Serum levels of OPN and CA125 in 52 patients with epithelium ovarian cancer,50 patients with epithelium benign ovarian tumor and 50 normal women as control group were measured by ELISA and chemilmninescentmicro particle immunoassay technology. Results The levels of OPN and CA125 in epithelium ovarian cancer were significantly higher than those in benign ovarian tumor and control group. The sensitivity and the specificity of OPN were 84.6% and 94.0%, respectively. The sensitivity and the specificity of CA125 were 75.0% and 90.0%, respectively. The sensitivity and the specificity of combined detection of two markers was 92.1% and 94.0%, respectively. Both serum OPN and CA125 levels of the preoperative epithelium ovarian cancer were higher than those of the postoperative epithelium of ovarian cancer. The OPN and CA125 levels of epithelium ovarian cancer stage Ⅲ were higher than those of stage Ⅰ and Ⅱ . Condusion OPN is an ideal turret marker and it can be used in diagnosing and monitoring of epithelium ovarian cancer. The clinical value can be increased by combined use of OPN and CA125.