结直肠癌患者手术前后血清sIL-2R和TNF水平的检测及临床意义

THE MEASUREMENT AND ITS CLINICAL SIGNIFICANCE OF THE LEVELS OF SERUM SOLUBLE INTERLEUKIN-2 RECEPTOR AND TUMOR NECROSIS FACTOR BEFORE, AND AFTER OPERATION IN PATIENTS WITH COLORECTAL CANCER

采用双抗体夹心ELISA法,对69例结直肠癌(CRC)患者手术前后血清可溶性白细胞介素-2受体(sIL-2R)和肿瘤坏死因子(TNF)水平进行了动态观察,结果表明:①结直肠癌患者血清sIL-2R和TNF水平均明显高于正常对照(P<0.01);②结直肠Dukes分期C期和D期的sIL-2R和TNF水平均明显高于A期和B期(P<0.05);③结直肠癌患者手术后1个月;血清sIL-2R和TNF水平均显著降低(P<0.05),④手术后1个月,行根治性手术者血清sIL-2R和TNF水平均明显降低(P<0.01),但仍比正常高;行姑息性手术者血清sIL-2R和TNF水平有所降低,但无显著差别(P>0.05),二组间有显著差异(P<0.05);⑤血清sIL-2R和TNF之间有显著的正相关性(r=0.374,P<0.01),二者并且对结直肠癌的临床分期、手术疗效评估及预后判断均有参考价值。

The preoperative and postoperative serum levels of soluble interleukin-2 receptor (sIL-2R) and tumor necrosis factor(TNF) weremeasured by ELISA in 69 patients with colorectal cancer (CRC). The tesults showed that:① the serum levels of sIL-2R and TNF in patients with CRC is significantly higher than that of normal control ( P < 0. 01 ) ;② the serum levels of sIL-2R and TNF in colorectal C and D stages wereelevated significantly than that of A and B stages ( P <0. 05) ;③ the serum levels of sIL-2R and TNF one month later in postopcration CRC patients were significantly decreased ( P <0. 05 ) ; ④ one month after operation , the serum sIL-2R and TNF levels were reduced significantly in CRC patients received cradical operation, but it is still higher than normal condition ; the levels of sIL-2R and TNF in patients receivedpaliative operation decreased, but no significant difference ( P >0. 05) ; there was significantly difference between the two groups ( P <0. 05). ㏕he serum sIL-2R is significantly associated with TNF ( r =0. 374, P <0. 01 ) , and both had reference value for classificantion of CRC, ealuation of the operation and judgement of prognosis.