摘要
目的:改进白及药典薄层色谱条件,并对白及薄层色谱中主要斑点化学成分进行分离鉴定。方法:采用色谱法进行化学成分分离,采用波谱法进行结构鉴定。结果:优化的薄层色谱条件为氯仿—甲醇—水(6:0.5:0.05);并分离得到4个主要化合物,分别为2,7-二羟基-4-甲氧基-9,10-二氢菲;4,4′-二甲氧基-9,9′10,10′-四氢-2,2′,7,7′-四羟基-1,1′-联菲;4,4′-二甲氧基-9,10-二氢-2,2′,7,7′-四羟基-1,1′-联菲;1-(4-羟基苄基)-2-甲氧基-4,7-二羟基-9,10-二氢菲。结论:优化了白及薄层色谱条件,并对主要色谱斑点化学成分进行了鉴定。
Objective: To improve the pharmacopoeia TCL method of Bletilla striata, and studies on the chemical constituents of the main spots in TLC. Methods: Isolation were carried out by chromatography, and identified by spectral data and compared with literature data. Result: The better method of thin layer chromatography was changed as CHCl3-MeOH-H2O (6: 0. 5:0. 05),and 4 compounds were isolated and identified as 4-methoxy-9,10-dihyd-rophenontrene-2,7-diol; 4,4'-dimethoxy-9,9',10,10'-tetrahydro- [1, 1'-biphenanthrene]-2,2', 7,7'-tetrol; 4,4'-dimethoxy-9,10-dihydro-[1, 1'-biphenanthrene]- 2,2', 7,7'-tetrol; 1-(4-hydroxybenzyl)-4-methoxy-9,10-dihydrophenontrene-2,7-diol. Conclusion: To improve TCL method of Bletilla striata, and the chemical constituents of the main spots in TLC were identified.
出处
《亚太传统医药》
2009年第9期23-25,共3页
Asia-Pacific Traditional Medicine
基金
北京工业大学博士启动基金项目(52015998200504)
关键词
白及
薄层色谱
化学成分
Bletilla striata
TCL
Chemical constituent